LOCUS       sequence-107146-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adenovirus for the expression of gRNAs targeting intron 19 of murine
            Alk and intron 14 of Eml4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han YC,
            Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura
            A
  TITLE     In vivo engineering of oncogenic chromosomal rearrangements with the
            CRISPR/Cas9 system.
  JOURNAL   Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 
            2014 Oct 22.
  PUBMED    25337876
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13902"; journalName: "Nature"; date: "2014-12-18- 
            18"; volume: "516"; issue: "7531"; pages: "423-7"
COMMENT     Sequence Label: Adeno EA
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             860..907
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(930..946)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(930..946)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac
       61 aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag
      121 aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc
      181 tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga
      241 gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct
      301 ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc
      361 cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa
      421 gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta
      481 tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca
      541 caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct
      601 ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat
      661 cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc
      721 cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt
      781 gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct
      841 gtctcagctg ggaggcgaca aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa
      901 gaaaaagtaa gaattcctgc agcccggggg atccactagt tctagagcgg ccgccaccgc
      961 ggggagatcc agacatgat
//
