LOCUS       sequence-107147-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used to characterize the promoter activities of PTet for dCas9 and 
            sgRNA response functions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Nielsen AA, Voigt CA
  TITLE     Multi-input CRISPR/Cas genetic circuits that interface host 
            regulatory networks.
  JOURNAL   Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
  PUBMED    25422271
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-24"; volume: "10:763. doi"; pages: " 
            10.15252/msb.20145735"
COMMENT     Sequence Label: pAN-PTet-YFP
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(136..387)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(178..199)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     527..546
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     786..807
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
       61 cgcgccgcca ttggccaaaa cgaaaaaagg cccccctttc gggaggcctc ttttctggaa
      121 tttggtaccg agttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
      181 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      241 gtagctaagg tagtggttgt cgggcagcag cacggggccg tcgccgattg gggtgttctg
      301 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
      361 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
      421 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      481 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
      541 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
      601 gaagtcgtgc agcttcatgt ggtcggggta gcgggcgaag cattgcaggc cgtagccgaa
      661 ggtggtcacg agggtgggcc agggcacggg cagcttgcct gtggtgcaga tgaacttcag
      721 ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc
      781 gtttacgtcg ccgtccagct cgaccaggat gg
//
