LOCUS       sequence-107161-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse 
            beta-Catenin. Expresses Cas9 from CBh promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Xue W, Chen S, Yin H, Tammela T, Papagiannakopoulos T, Joshi NS, Cai
            W, Yang G, Bronson R, Crowley DG, Zhang F, Anderson DG, Sharp PA, 
            Jacks T
  TITLE     CRISPR-mediated direct mutation of cancer genes in the mouse liver.
  JOURNAL   Nature. 2014 Aug 6. doi: 10.1038/nature13589.
  PUBMED    25119044
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Aug 6. doi: 10.1038/nature13589."
COMMENT     Sequence Label: pX330 Ctnnb1.2
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        120..368
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     120..140
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     291..310
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        390..465
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        562..847
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
       61 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtg
      121 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
      181 taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
      241 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
      301 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      361 gaaacaccga gctccttccc tgagtggcag ttttagagct agaaatagca agttaaaata
      421 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag
      481 ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag
      541 acaaatggct ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc
      601 gcccaacgac ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg
      661 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      721 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca
      781 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      841 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
      901 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
      961 gggggggggg g
//
