LOCUS       sequence-107168-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible; gRNA seq is ATGAGAATCAAGGCGGTCGA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Moore R, Spinhirne A, Lai MJ, Preisser S, Li Y, Kang T, Bleris L
  TITLE     CRISPR-based self-cleaving mechanism for controllable gene delivery 
            in human cells.
  JOURNAL   Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 
            10.1093/nar/gku1326. Epub 2014 Dec 18.
  PUBMED    25527740
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1326"; journalName: "Nucleic Acids Res"; date: 
            "2015-01-30- 30"; volume: "43"; issue: "2"; pages: "1297-303"
COMMENT     Sequence Label: ptreCas9-mKate2ps-T1gRNA
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      60..69
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 caacactttt gtcttatacc aactttccgg ctagtggatc cgcggccgtt actagccttg
       61 ccaccatgga caagaagtac tccattgggc tcgatatcgg cacaaacagc gtcggctggg
      121 ccgtcattac ggacgagtac aaggtgccga gcaaaaaatt caaagttctg ggcaataccg
      181 atcgccacag cataaagaag aacctcattg gcgccctcct gttcgactcc ggggagacgg
      241 ccgaagccac gcggctcaaa agaacagcac ggcgcagata tacccgcaga aagaatcgga
      301 tctgctacct gcaggagatc tttagtaatg agatggctaa ggtggatgac tctttcttcc
      361 ataggctgga ggagtccttt ttggtggagg aggataaaaa gcacgagcgc cacccaatct
      421 ttggcaatat cgtggacgag gtggcgtacc atgaaaagta cccaaccata tatcatctga
      481 ggaagaagct tgtagacagt actgataagg ctgacttgcg gttgatctat ctcgcgctgg
      541 cgcatatgat caaatttcgg ggacacttcc tcatcgaggg ggacctgaac ccagacaaca
      601 gcgatgtcga caaactcttt atccaactgg ttcagactta caatcagctt ttcgaagaga
      661 acccgatcaa cgcatccgga gttgacgcca aagcaatcct gagcgctagg ctgtccaaat
      721 cccggcggct cgaaaacctc atcgcacagc tccctgggga gaagaagaac ggcctgtttg
      781 gtaatcttat cgccctgtca ctcgggctga cccccaactt taaatctaac ttcgacctgg
      841 ccgaagatgc caagcttcaa ctgagcaaag acacctacga tgatgatctc gacaatctgc
      901 tggcccagat cggcgaccag tacgcagacc tttttt
//
