LOCUS       sequence-107170-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible; gRNA seq is ATGAGAATCAAGGCGGTCGA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Moore R, Spinhirne A, Lai MJ, Preisser S, Li Y, Kang T, Bleris L
  TITLE     CRISPR-based self-cleaving mechanism for controllable gene delivery 
            in human cells.
  JOURNAL   Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 
            10.1093/nar/gku1326. Epub 2014 Dec 18.
  PUBMED    25527740
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1326"; journalName: "Nucleic Acids Res"; date: 
            "2015-01-30- 30"; volume: "43"; issue: "2"; pages: "1297-303"
COMMENT     Sequence Label: ptreCas9-mKate2ps-T1gRNA
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      305..314
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(390..414)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    459..477
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    495..513
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    531..549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    567..585
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    603..621
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    639..657
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    675..693
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tcctccagcc tatggaagaa agagtcatcc accttagcca tctcattact aaagatctcc
       61 tgcaggtagc agatccgatt ctttctgcgg gtatatctgc gccgtgctgt tcttttgagc
      121 cgcgtggctt cggccgtctc cccggagtcg aacaggaggg cgccaatgag gttcttcttt
      181 atgctgtggc gatcggtatt gcccagaact ttgaattttt tgctcggcac cttgtactcg
      241 tccgtaatga cggcccagcc gacgctgttt gtgccgatat cgagcccaat ggagtacttc
      301 ttgtccatgg tggcaaggct agtaacggcc gcggatccac tagccggaaa gttggtataa
      361 gacaaaagtg ttgtggaatt gctccaggcg atctgacggt tcactaaacg agctctgctt
      421 ttataggcgc ccaccgtaca cgcctaaagc ttatacgttc tctatcactg atagggagta
      481 aactggatat acgttctcta tcactgatag ggagtaaact gtagatacgt tctctatcac
      541 tgatagggag taaactggtc atacgttctc tatcactgat agggagtaaa ctccttatac
      601 gttctctatc actgataggg agtaaagtct gcatacgttc tctatcactg atagggagta
      661 aactcttcat acgttctcta tcactgatag ggagtaaact cgagtatgtc gaggtggcgt
      721 gtacggggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      781 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      841 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      901 cccgacagga ctata
//
