LOCUS       sequence-107172-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KCTD13 N-terminus disruption by homolgy-directed repair.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Martinez RA, Stein JL, Krostag AR, Nelson AM, Marken JS, Menon V, 
            May RC, Yao Z, Kaykas A, Geschwind DH, Grimley JS
  TITLE     Genome engineering of isogenic human ES cells to model autism 
            disorders.
  JOURNAL   Nucleic Acids Res. 2015 Mar 11. pii: gkv164.
  PUBMED    25765640
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Mar 11. pii: gkv164."
COMMENT     Sequence Label: AI-CN590
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..32
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             84..140
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
       61 acgagctgta caagggaagc ggagctacta acttcagcct gctgaagcag gctggagacg
      121 tggaggagaa ccctggacct actagtatga ctgaatacaa acccactgtc cgactggcaa
      181 caagagatga tgtcccaaga gccgtgagaa cactggcagc agctttcgcc gactacccag
      241 ccacaagaca cactgtggac cccgatcggc atatcgagag agtgaccgag ctgcaggaac
      301 tgttcctgac aagggtgggc ctggacattg ggaaggtctg ggtcgcagac gatggagcag
      361 ctgtggctgt ctggaccaca ccagagagcg tggaagccgg agctgtcttt gcagagatcg
      421 gccctcgaat ggcagaactg agcggctccc ggctggcagc acagcagcag atggaggggc
      481 tgctggcccc acaccgccct aaggaaccag catggttcct ggccactgtg ggagtctctc
      541 ccgatcatca gggcaaaggg ctgggaagtg ctgtggtcct gcctggggtg gaggctgcag
      601 aacgagctgg agtcccagca tttctggaga catctgcccc caggaacctg cctttctatg
      661 aacgcctggg ctttactgtg accgccgatg tggaagtgcc cgagggaccc cgaacctggt
      721 gtatgacccg aaagcctgga gcctaataga ccggtactcc tcaggtgcag gctgcctatc
      781 agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
//
