LOCUS       sequence-107174-         638 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AdEasy Shuttle vector modified for multicistronic expression. 
            Contains T2A, F2A and Venus tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 638)
  TITLE     Modified AdEasy shuttle vectors for multicistronic expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pShuttle-CMV-F2A-T2A-Venus
FEATURES             Location/Qualifiers
     source          1..638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(150..401)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(192..213)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     541..560
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
       61 aaacctctac aaatgtggta tggctgatta tgatcagtta tctagatccg gtggatcgga
      121 tatcttatct agaagcttgc tcattactac ttgtacagct cgtccatgcc gagagtgatc
      181 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
      241 ctcagggcgg actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
      301 atgggggtgt tctgctggta gtggtcggcg agctgcacgc cgccgtcctc gatgttgtgg
      361 cggatcttga agttggcctt gatgccgttc ttctgcttgt cggcggtgat atagacgttg
      421 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
      481 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
      541 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
      601 atggcggact tgaagaagtc gtgctgcttc atgtggtc
//
