LOCUS       sequence-107175-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AdEasy Shuttle vector modified for multicistronic expression. 
            Contains T2A and F2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  TITLE     Modified AdEasy shuttle vectors for multicistronic expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pShuttle-CMV-F2A-T2A
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..123
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             154..207
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    366..487
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(403..422)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     457..476
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agtgaaccgt cagatccgct agagatctgg taccgtcgac gcggccgcgg aagcggagtg
       61 aaacagactt tgaattttga ccttctcaag ttggcgggag acgtggagtc caaccctgga
      121 cctaggcctg gaagcggaac gcgtggaagc ggagagggca gaggaagtct gctaacatgc
      181 ggtgacgtcg aggagaatcc tggacctctc gagcctaagc ttctagataa gatatccgat
      241 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
      301 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
      361 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      421 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
      481 tatcttaacg cggatctggg cgtggttaag ggtgggaaag aatatataag gtgggggtct
      541 tatgtagttt tgtatctgtt ttgcagcagc cgccgccgcc atgagcacca actcgtttga
      601 tggaagcatt gtgagctcat atttgacaac gcgcatgccc ccatgggccg gggtgcgtca
      661 gaatgtgatg ggctccagca ttgatggtcg ccccgtcctg
//
