LOCUS       sequence-107176-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AdEasy Shuttle vector modified for multicistronic expression. 
            Contains T2A and F2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  TITLE     Modified AdEasy shuttle vectors for multicistronic expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pShuttle-CMV-F2A-T2A
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(148..201)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(232..297)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     promoter        complement(360..563)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(389..409)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
       61 aaacctctac aaatgtggta tggctgatta tgatcagtta tctagatccg gtggatcgga
      121 tatcttatct agaagcttag gctcgagagg tccaggattc tcctcgacgt caccgcatgt
      181 tagcagactt cctctgccct ctccgcttcc acgcgttccg cttccaggcc taggtccagg
      241 gttggactcc acgtctcccg ccaacttgag aaggtcaaaa ttcaaagtct gtttcactcc
      301 gcttccgcgg ccgcgtcgac ggtaccagat ctctagcgga tctgacggtt cactaaacca
      361 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
      421 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
      481 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
      541 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
      601 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
      661 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagt
//
