LOCUS       sequence-107181-         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of GFP T2A Cre fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Foijer F, Xie SZ, Simon JE, Bakker PL, Conte N, Davis SH, Kregel E, 
            Jonkers J, Bradley A, Sorger PK
  TITLE     Chromosome instability induced by Mps1 and p53 mutation generates 
            aggressive lymphomas exhibiting aneuploidy-induced stress.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13427-32. doi: 
            10.1073/pnas.1400892111. Epub 2014 Sep 2.
  PUBMED    25197064
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1400892111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-09-16- 16"; volume: "111"; issue: "37"; pages: 
            "13427-32"
COMMENT     Sequence Label: pRetroX GFP T2A Cre
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..70
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cggcggctcc ggcggcgagg gcagaggctc cctgctgacc tgcggcgacg tggaggagaa
       61 ccccggcccc ggatctatgg ccaatttact gaccgtacac caaaatttgc ctgcattacc
      121 ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca gggatcgcca
      181 ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg
      241 gtgcaagttg aataaccgga aatggtttcc cgcagaacct gaagatgttc gcgattatct
      301 tctatatctt caggcgcgcg gtctggcagt aaaaactatc cagcaacatt tgggccagct
      361 aaacatgctt catcgtcggt ccgggctgcc acgaccaagt gacagcaatg ctgtttcact
      421 ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct
      481 agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata gcgatcgctg
      541 ccaggatata cgtaatctgg catttctggg gattgcttat aacaccctgt tacgtatagc
      601 cgaaattgcc aggatcaggg ttaaagatat ctcacgtact gacggtggga gaatgttaat
      661 ccatattggc agaacg
//
