LOCUS       sequence-107183-        1103 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: PX854
FEATURES             Location/Qualifiers
     source          1..1103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      56..65
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             68..88
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             113..433
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 tgtttaatta cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac
       61 catggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag ccggcgtgca
      121 ggtggagacc atcagccccg gcgacggcag aaccttcccc aagagaggcc agacctgcgt
      181 ggtgcactac accggcatgc tggaggacgg caagaagttc gacagcagca gagacagaaa
      241 caagcccttc aagttcatgc tgggcaagca ggaggtgatc agaggctggg aggagggcgt
      301 ggcccagatg agcgtgggcc agagagccaa gctgaccatc agccccgact acgcctacgg
      361 cgccaccggc caccccggca tcatccctcc tcacgccacc ctggtgttcg acgtggagct
      421 gctgaagctg gagggtggcg gtggctcggg tggcggtggc tcgggtggcg gtggctcgtg
      481 cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata
      541 ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga
      601 cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga
      661 acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg
      721 gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca
      781 gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat
      841 gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc
      901 cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa
      961 gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca
     1021 caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca
     1081 gaagaacagc cgcgagagaa tga
//
