LOCUS       sequence-107187-        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-term SpCas9 piece of inducible transcriptional activator 
            (dCas9(C)-FKBP-2xNLS-VP64).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: PX856
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             67..87
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             112..432
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 gtttaattac ctggagcacc tgcctgaaat cacttttttt caggttggac cggtgccacc
       61 atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc cggcgtgcag
      121 gtggagacca tcagccccgg cgacggcaga accttcccca agagaggcca gacctgcgtg
      181 gtgcactaca ccggcatgct ggaggacggc aagaagttcg acagcagcag agacagaaac
      241 aagcccttca agttcatgct gggcaagcag gaggtgatca gaggctggga ggagggcgtg
      301 gcccagatga gcgtgggcca gagagccaag ctgaccatca gccccgacta cgcctacggc
      361 gccaccggcc accccggcat catccctcct cacgccaccc tggtgttcga cgtggagctg
      421 ctgaagctgg agggtggcgg tggctcgggt ggcggtggct cgggtggcgg tggctcgaag
      481 cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac
      541 cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac
      601 tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat
      661 ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg
      721 gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg
      781 aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac
      841 accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc
      901 aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg
      961 atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag
     1021 ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc
     1081 atcctgcaga c
//
