LOCUS       sequence-107189-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-term SpCas9 piece of inducible transcriptional activator 
            (dCas9(C)-FKBP-2xNLS-VP64).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: PX856
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        126..374
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     126..146
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     297..316
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        393..468
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        565..850
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
       61 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      121 catgtgaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
      181 gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
      241 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
      301 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      361 aaggacgaaa caccgggtct tcgagaagac ctgttttaga gctagaaata gcaagttaaa
      421 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgtttta
      481 gagctagaaa tagcaagtta aaataaggct agtccgtttt tagcgcgtgc gccaattctg
      541 cagacaaatg gctctagagg tacccgttac ataacttacg gtaaatggcc cgcctggctg
      601 accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga ctttccattg
      661 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      721 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattgtgc
      781 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      841 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
      901 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
      961 gggggggggg g
//
