LOCUS       sequence-107190-        1139 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-term SpCas9 piece of inducible transcriptional activator 
            (dCas9(C)-FKBP-2xNLS-VP64).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149."
COMMENT     Sequence Label: PX856
FEATURES             Location/Qualifiers
     source          1..1139
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..165)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(184..204)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gaattcttag ttaatcagca tgtccaggtc gaaatcatca agggcgtcac tgccgagcat
       61 gtcgaggtca aagtcatcaa gggcatccga accaagcatg tcaaggtcaa aatcatcgag
      121 ggcgtcactt cccagcatat ccagatcaaa atcgtccaat gcgtcagccc gtccgctggc
      181 ctccaccttt ctcttcttct tggggctgtc gcctcccagc tgagacaggt cgatccgtgt
      241 ctcgtacagg ccggtgatgc tctggtggat cagggtggcg tccagcacct ctttggtgct
      301 ggtgtacctc ttccggtcga tggtggtgtc aaagtacttg aaggcggcag gggctcccag
      361 attggtcagg gtaaacaggt ggatgatatt ctcggcctgc tctctgatgg gcttatcccg
      421 gtgcttgttg taggcggaca gcactttgtc cagattagcg tcggccagga tcactctctt
      481 ggagaactcg ctgatctgct cgatgatctc gtccaggtag tgcttgtgct gttccacaaa
      541 cagctgtttc tgctcattat cctcggggga gcccttcagc ttctcatagt ggctggccag
      601 gtacaggaag ttcacatatt tggagggcag ggccagttcg tttcccttct gcagttcgcc
      661 ggcagaggcc agcattctct tccggccgtt ttccagctcg aacagggagt acttaggcag
      721 cttgatgatc aggtcctttt tcacttcttt gtagcccttg gcttccagaa agtcgatggg
      781 attcttctcg aagctgcttc tttccatgat ggtgatcccc agcagctctt tcacactctt
      841 cagtttcttg gacttgccct tttccacttt ggccaccacc agcacagaat aggccacggt
      901 ggggctgtcg aagccgccgt acttcttagg gtcccagtcc ttctttctgg cgatcagctt
      961 atcgctgttc ctcttgggca ggatagactc tttgctgaag ccgcctgtct gcacctcggt
     1021 ctttttcacg atattcactt ggggcatgct cagcactttc cgcacggtgg caaaatcccg
     1081 gcccttatcc cacacgatct ccccggtttc gccgtttgtc tcgatcagag gccgcttcc
//
