LOCUS       sequence-107196-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Episome vector for Phaeodactylum tricornutum (pCC1BAC-based).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Karas BJ, Diner RE, Lefebvre SC, McQuaid J, Phillips AP, Noddings 
            CM, Brunson JK, Valas RE, Deerinck TJ, Jablanovic J, Gillard JT, 
            Beeri K, Ellisman MH, Glass JI, Hutchison CA 3rd, Smith HO, Venter 
            JC, Allen AE, Dupont CL, Weyman PD
  TITLE     Designer diatom episomes delivered by bacterial conjugation.
  JOURNAL   Nat Commun. 2015 Apr 21;6:6925. doi: 10.1038/ncomms7925.
  PUBMED    25897682
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-04-21"; volume: "6:6925. doi"; pages: " 
            10.1038/ncomms7925"
COMMENT     Sequence Label: p0521s
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..35
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     27..44
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     28..44
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    72..128
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(171..187)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(171..187)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(184..206)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    195..211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(219..225,226..243,244..249))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    264..285
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact
       61 cactataggg cgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat
      121 gcaagcttga gtattctata gtctcaccta aatagcttgg cgtaatcatg gtcatagctg
      181 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      241 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      301 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      361 gaaccccttg cggccgcccg ggccgtcgac caattctcat gtttgacagc ttatcatcga
      421 atttctgcca ttcatccgct tattatcact tattcaggcg tagcaaccag gcgtttaagg
      481 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
      541 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
      601 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
      661 ggcgaagaag ttgtccatat tggccacg
//
