LOCUS       sequence-107211-         481 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal His tagged round 3 aldehyde-resistant GUS expression 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Matsumura I, Wallingford JB, Surana NK, Vize PD, Ellington AD
  TITLE     Directed evolution of the surface chemistry of the reporter enzyme 
            beta-glucuronidase.
  JOURNAL   Nat Biotechnol. 1999 Jul;17(7):696-701.
  PUBMED    10404164
REFERENCE   2  (bases 1 to 481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol."; date: "1999-07"; volume: "17(7)"; pages: "696-701"
COMMENT     Sequence Label: 6his R.3-AR GUSpET28a+/pLysS
FEATURES             Location/Qualifiers
     source          1..481
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgttacgt cctgtagaaa ccccaacccg tgaaatcaaa
      121 aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa actgtggaat tgatcagcgt
      181 tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg tgccaggcag ttttaacgat
      241 cagttcgccg atgcagatat tcgtaattat gcgggcgacg tctggtatca gcgcgaagtc
      301 tttataccaa aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc ggtcactcat
      361 tacggcaaag tgtgggtcaa taatcaagaa gtgatggagc atcagggcgg ctatacgcca
      421 tttgaggccg atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtat caccgtttgt
      481 g
//
