LOCUS       sequence-107227-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mm-cpn cysteine double mutant for crosslinking.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Hoersch D, Roh SH, Chiu W, Kortemme T
  TITLE     Reprogramming an ATP-driven protein machine into a light-gated 
            nanocage.
  JOURNAL   Nat Nanotechnol. 2013 Dec;8(12):928-32. doi: 10.1038/nnano.2013.242.
            Epub 2013 Nov 24.
  PUBMED    24270642
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nnano.2013.242"; journalName: "Nat Nanotechnol"; date: 
            "2013-12"; volume: "8"; issue: "12"; pages: "928-32"
COMMENT     Sequence Label: c7-87/199_HLL
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    62..86
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..123
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
       61 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacat atgtcacaac aaccaggagt tttacctgaa aacatgaaaa gatacatggg
      181 aagagatgct caaagaatga acatcttagc aggaagaatt atcgcagaaa cagtaagatc
      241 aacactcggt ccaaaaggaa tggacaaaat gttagttgac gacttagggg acgttgttgt
      301 tacaaacgac ggtgttacaa tcttaagaga aatgagtgta gaacaccctg cagctaaaat
      361 gttaatcgaa gttgcaaaaa cacaagaatg cgaagttggc gacggtacaa caacagctgt
      421 tgtagttgct ggtgaattat taagaaaagc tgaagaatta ttggaccaaa atgtacaccc
      481 aacaattgtt gtaaaaggat accaagcagc agctcaaaaa gctcaagaat tattaaaaac
      541 aattgcgtcc gaagttggag ctcaagacaa agaaatctta acaaaaatcg caatgacctc
      601 aatcaccgga aaaggtgctg aaaaagctaa agaaaaatta gctgaaatca ttgttgaagc
      661 agtttcagca gttgttgacg acgaaggaaa agttgacaaa gatttaatca aaattgagaa
      721 aa
//
