LOCUS       sequence-107235-         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cysteine free Mm-cpn mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Hoersch D, Roh SH, Chiu W, Kortemme T
  TITLE     Reprogramming an ATP-driven protein machine into a light-gated 
            nanocage.
  JOURNAL   Nat Nanotechnol. 2013 Dec;8(12):928-32. doi: 10.1038/nnano.2013.242.
            Epub 2013 Nov 24.
  PUBMED    24270642
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nnano.2013.242"; journalName: "Nat Nanotechnol"; date: 
            "2013-12"; volume: "8"; issue: "12"; pages: "928-32"
COMMENT     Sequence Label: c7_HLL
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    64..88
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             103..125
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac gactcactat
       61 aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttaagaa
      121 ggagatatac atatgtcaca acaaccagga gttttacctg aaaacatgaa aagatacatg
      181 ggaagagatg ctcaaagaat gaacatctta gcaggaagaa ttatcgcaga aacagtaaga
      241 tcaacactcg gtccaaaagg aatggacaaa atgttagttg acgacttagg ggacgttgtt
      301 gttacaaacg acggtgttac aatcttaaga gaaatgagtg tagaacaccc tgcagctaaa
      361 atgttaatcg aagttgcaaa aacacaagaa aaagaagttg gcgacggtac aacaacagct
      421 gttgtagttg ctggtgaatt attaagaaaa gctgaagaat tattggacca aaatgtacac
      481 ccaacaattg ttgtaaaagg ataccaagca gcagctcaaa aagctcaaga attattaaaa
      541 acaattgcgt ccgaagttgg agctcaagac aaagaaatct taacaaaaat cgcaatgacc
      601 tcaatcaccg gaaaaggtgc tgaaaaagct aaagaaaaat tagctgaaat cattgttgaa
      661 gcagtttcag cagttgttga cgacgaagga a
//
