LOCUS       sequence-107248-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV115.loxP
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..57
                     /label=loxPsym
                     /bound_moiety="Cre recombinase"
                     /note="loxPsym has a symmetric spacer region, and loxPsym 
                     sites are capable of recombining in either orientation 
                     (Hoess et al., 1986)."
     CDS             complement(129..806)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             813..824
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    842..858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(866..872,873..890,891..896))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 cccctcgagg tcgacggcag ctgataactt cgtataatgt acattatacg aagttatggg
       61 acgacctgca gcccccagta gagaccgcct ggctctagta gcgatctaca ctagcactat
      121 cagcgttatt aagcaccggt ggagtgacga ccttcagcac gttcgtactg ttcaacgatg
      181 gtgtagtctt cgttgtggga ggtgatgtcc agtttgatgt cggttttgta agcacccggc
      241 agctgaaccg gttttttagc catgtaggtg gttttaactt cagcgtcgta gtgaccaccg
      301 tctttcagtt tcagacgcat tttgatttca cctttcagag caccgtcttc cgggtacata
      361 cgttcggtgg aagcttccca acccatggtt tttttctgca taaccggacc gtcggacggg
      421 aagttggtac cacgcagttt aactttgtag atgaactcac cgtcttgcag ggaggagtcc
      481 tgggtaacgg taacaacacc accgtcttcg aagttcataa cacgttccca tttgaaacct
      541 tccgggaagg acagtttcag gtagtccggg atgtcagccg ggtgtttaac gtaagctttg
      601 gaaccgtact ggaactgcgg ggacaggatg tcccaagcga acggcagcgg accacctttg
      661 gtaactttca gtttagcggt ctgggtacct tcgtacggac gaccttcacc ttcaccttcg
      721 atttcgaact cgtgaccgtt aacggaacct tccatacgaa ctttgaaacg catgaactct
      781 ttgataacgt cttcggagga agccatctag tatttctcct ctttctctag tatgtgtgaa
      841 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      901 gg
//
