LOCUS       sequence-107249-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MCP-VP64 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC522
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             161..181
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             554..703
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 gctgtcttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat
       61 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag
      121 catacaatca actatctcat atacaatgtc tctcgagatg cccaaaaaga aaagaaaagt
      181 gggtagtatg gcttctaact ttactcagtt cgttctcgtc gacaatggcg gaactggcga
      241 cgtgactgtc gccccaagca acttcgctaa cgggatcgct gaatggatca gctctaactc
      301 gcgttcacag gcttacaaag taacctgtag cgttcgtcag agctctgcgc agaatcgcaa
      361 atacaccatc aaagtcgagg tgcctaaagg cgcctggcgt tcgtacttaa atatggaact
      421 aaccattcca attttcgcca cgaattccga ctgcgagctt attgttaagg caatgcaagg
      481 tctcctaaaa gatggaaacc cgattccctc agcaatcgca gcaaactccg gcatctacgg
      541 ctccggacga gccgatgctc tcgacgattt tgacctcgat atgctgggat ccgacgctct
      601 cgatgacttc gaccttgaca tgctggggag tgacgctctg gacgacttcg atctcgacat
      661 gctgggctcc gacgccctgg atgacttcga tctggacatg ctcatcaact gagcggccgc
      721 caccgcggtg gagctctaag c
//
