LOCUS       sequence-107257-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS404 backbone, contains an RFP flanked by BsaI sites with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV104
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..22
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7..23
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(107..784)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             791..802
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    820..836
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(844..850,851..868,869..874))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ccccctcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccccagtaga
       61 gaccgcctgg ctctagtagc gatctacact agcactatca gcgttattaa gcaccggtgg
      121 agtgacgacc ttcagcacgt tcgtactgtt caacgatggt gtagtcttcg ttgtgggagg
      181 tgatgtccag tttgatgtcg gttttgtaag cacccggcag ctgaaccggt tttttagcca
      241 tgtaggtggt tttaacttca gcgtcgtagt gaccaccgtc tttcagtttc agacgcattt
      301 tgatttcacc tttcagagca ccgtcttccg ggtacatacg ttcggtggaa gcttcccaac
      361 ccatggtttt tttctgcata accggaccgt cggacgggaa gttggtacca cgcagtttaa
      421 ctttgtagat gaactcaccg tcttgcaggg aggagtcctg ggtaacggta acaacaccac
      481 cgtcttcgaa gttcataaca cgttcccatt tgaaaccttc cgggaaggac agtttcaggt
      541 agtccgggat gtcagccggg tgtttaacgt aagctttgga accgtactgg aactgcgggg
      601 acaggatgtc ccaagcgaac ggcagcggac cacctttggt aactttcagt ttagcggtct
      661 gggtaccttc gtacggacga ccttcacctt caccttcgat ttcgaactcg tgaccgttaa
      721 cggaaccttc catacgaact ttgaaacgca tgaactcttt gataacgtct tcggaggaag
      781 ccatctagta tttctcctct ttctctagta tgtgtgaaat tgttatccgc tcacaattcc
      841 acacaacata cgagccggaa gcataaagtg taaagcctgg
//
