LOCUS       sequence-107261-         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  COM-VP64 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC506
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             161..181
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             389..538
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 gctgtcttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat
       61 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag
      121 catacaatca actatctcat atacaatgtc tctcgagatg cccaaaaaga aaagaaaagt
      181 gggtagtatg aaatcaattc gctgtaaaaa ctgcaacaaa ctgttattta aggcggatag
      241 ttttgatcac attgaaatca ggtgtccgcg ttgcaaacgt cacatcataa tgctgaatgc
      301 ctgcgagcat cccacggaga aacattgtgg gaaaagagaa aaaatcacgc attctgacga
      361 aaccgtgcgt tatggctccg gacgagccga tgctctcgac gattttgacc tcgatatgct
      421 gggatccgac gctctcgatg acttcgacct tgacatgctg gggagtgacg ctctggacga
      481 cttcgatctc gacatgctgg gctccgacgc cctggatgac ttcgatctgg acatgctcat
      541 caactgagcg gccgccaccg cggtggagct ctaagcaaat agctaaatta tatacgaatt
      601 aatattatga ttaagtgttt acgtgagtgc gatattttta ttactatctt atacagttgt
      661 atatactcta t
//
