LOCUS       sequence-107263-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.F3.loxP
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    112..145
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      668..725
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(749..766)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
       61 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggtctcattt tataacttcg
      121 tataatgtat gctatacgaa gttatactag tgcatgcaga aacttctgtg gcttgacaga
      181 taaataactg cagtagtcgg tgcgtactaa ttgtttggtc gtgtctgaaa aatcttgaat
      241 tttcagaaaa gaataagccc caaatgtcag tgatggtagt agcagtactc ccctacgatt
      301 ttagatactt tagagagccc accttcagaa tcggaaggag gataattttg taaagccctt
      361 ctgttttttc tcttgcataa cttatatttc cacatcaaaa agtagtgtgc taagaaaaag
      421 gtcacaagaa aaaggattac ggcactctct gcatctagac atataccaaa agttgggttt
      481 gctcacgaaa ataccataat tgtggtgtca aaaaaatcct gcctcataat accactgcag
      541 caattgtgga tgactaaaaa ataacttgca ttccacgatg ttattttact ttataaagca
      601 cctgcaattt ttttttttgt attaactcat cgagtatgtc tgatgatggg atacgcggcc
      661 gctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
      721 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
      781 ggctgcggcg agcggtatca gctcactcaa
//
