LOCUS       sequence-107268-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.K6.loxP
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    117..150
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      689..746
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(770..787)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
       61 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggtct cattttataa
      121 cttcgtataa tgtatgctat acgaagttat actagtgcat gctttggaca gtgataccga
      181 agaaggaagt aaattcagta gcgccgcatg aggtagtagc agaaggagtt aaggttgacc
      241 atggctcact tggcatatac tgttgggcag aaacagtata gtgaaactgt tatcaaacca
      301 atatatttgg gctacatggc tttcctttcc cgaaaaagac accatttagt aattatgttg
      361 tacttttact ctctgaactt ctgtgaatat aaaaaatata ctaaataaaa ggatgcaact
      421 tccttaaaga gggcaagata gcgacataaa gtggaaaagt gttagcaaca ttgcgcaggc
      481 atcgattaat atatgcatat atacttttta cgtagtctac gttaaattgt caatagatca
      541 agcagaatcg gtagctctgt taagcgatta agcccagtgg ctttttgtcg caccgtttag
      601 gttcgcgtta gcaagatgga aaaaaaagaa cgcgaatcat gtattgacgt atgacgtatt
      661 aggcagatgg gatacgcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct
      721 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg
      781 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      841 tacggttatc cacagaatca gg
//
