LOCUS       sequence-107269-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.K5.loxP
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    112..145
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      684..741
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(765..782)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
       61 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggtctcattt tataacttcg
      121 tataatgtat gctatacgaa gttatactag tgcatgcttt ggacagtgat accgaagaag
      181 gaagtaaatt cagtagcgcc gcatgaggta gtagcagaag gagttaaggt tgaccatggc
      241 tcacttggca tatactgttg ggcagaaaca gtatagtgaa actgttatca aaccaatata
      301 tttgggctac atggctttcc tttcccgaaa aagacaccat ttagtaatta tgttgtactt
      361 ttactctctg aacttctgtg aatataaaaa atatactaaa taaaaggatg caacttcctt
      421 aaagagggca agatagcgac ataaagtgga aaagtgttag caacattgcg caggcatcga
      481 ttaatatatg catatatact ttttacgtag tctacgttaa attgtcaata gatcaagcag
      541 aatcggtagc tctgttaagc gattaagccc agtggctttt tgtcgcaccg tttaggttcg
      601 cgttagcaag atggaaaaaa aagaacgcga atcatgtatt gacgtatgac gtattaggca
      661 gatgggatac gcggccgctg cagtccggca aaaaagggca aggtgtcacc accctgccct
      721 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
      781 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      841 ttatccacag aatcagg
//
