LOCUS       sequence-107273-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.HO5.loxP
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    112..145
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      681..738
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(762..779)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
       61 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggtctcattt tataacttcg
      121 tataatgtat gctatacgaa gttatactag tgcatgcttt aaagtataga tagaattgat
      181 tgctgcttat gaggatatgg atttagagct aatagttgta atagaaataa aagccattta
      241 gaataggaat tgaatacaat tttatgagct ttaaatattt aatgatgaat agtgcgttta
      301 gaacgcttca tcatgcttca acaagcttct gcaaacctca tttgagtagc aatagctgtt
      361 tacttttttc cgcgacttta ttcgaagtta tggtgaactt ttcgtggttt cacacctaat
      421 aacgcccagc ggtagtacaa ttcaaagtag taggtaccaa tggtagtact agtgggtgat
      481 agtagcaaat ttctctatga aaatgaattg ttgctctgca atgatgttaa tgtggacatg
      541 acatgaattt tcgcgatatg ttgatgtaaa taataacatg attttcgtgg atcatttcgt
      601 aaaccatagg tttatttcgt gaattactaa gagaaaggca tagaaacagg acttgcggat
      661 gggatacgcg gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt
      721 tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg
      781 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      841 tccacagaat cagg
//
