LOCUS       sequence-107275-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.KH
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      650..707
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(731..748)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
       61 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggtctcattt tactagtgca
      121 tgctttggac agtgataccg aagaaggaag taaattcagt agcgccgcat gaggtagtag
      181 cagaaggagt taaggttgac catggctcac ttggcatata ctgttgggca gaaacagtat
      241 agtgaaactg ttatcaaacc aatatatttg ggctacatgg ctttcctttc ccgaaaaaga
      301 caccatttag taattatgtt gtacttttac tctctgaact tctgtgaata taaaaaatat
      361 actaaataaa aggatgcaac ttccttaaag agggcaagat agcgacataa agtggaaaag
      421 tgttagcaac attgcgcagg catcgattaa tatatgcata tatacttttt acgtagtcta
      481 cgttaaattg tcaatagatc aagcagaatc ggtagctctg ttaagcgatt aagcccagtg
      541 gctttttgtc gcaccgttta ggttcgcgtt agcaagatgg aaaaaaaaga acgcgaatca
      601 tgtattgacg tatgacgtat taggcagatg ggatacgcgg ccgctgcagt ccggcaaaaa
      661 agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta
      721 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      781 cagctcactc aaaggcggta atacggttat ccacagaatc agg
//
