LOCUS       sequence-107276-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human immunoglobulin epsilon antibody expression vector (kappa light
            chain) without variable regions. Enables Colony-PCR screening of 
            false-positive (PCR vector template) colonies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, 
            Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
  TITLE     A tool kit for rapid cloning and expression of recombinant 
            antibodies.
  JOURNAL   Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885.
  PUBMED    25073855
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-07-30"; volume: "4:5885. doi"; pages: " 
            10.1038/srep05885"
COMMENT     Sequence Label: pVITRO1-dV-IgE/kappa
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    446..890
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        923..969
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        961..979
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agccaccgct aattcaaagc aatccggaat gttgccatca caactcattg ggtttctgct
       61 gctctgggtt ccagctagcc gcggtgaatt cgatatcctc gagcgtacgg tggcggcgcc
      121 atctgtcttc atcttcccgc catctgatga gcagttgaaa tctggaactg cctctgttgt
      181 gtgcctgctg aataacttct atcccagaga ggccaaagta cagtggaagg tggataacgc
      241 cctccaatcg ggtaactccc aggagagtgt cacagagcag gacagcaagg acagcaccta
      301 cagcctcagc agcaccctga cgctgagcaa agcagactac gagaaacaca aagtctacgc
      361 ctgcgaagtc acccatcagg gcctgagctc gcccgtcaca aagagcttca acaggggaga
      421 gtgttaggga tcccgtacgc ctaggagcag gtttccccaa tgacacaaaa cgtgcaactt
      481 gaaactccgc ctggtctttc caggtctaga ggggtaacac tttgtactgc gtttggctcc
      541 acgctcgatc cactggcgag tgttagtaac agcactgttg cttcgtagcg gagcatgacg
      601 gccgtgggaa ctcctccttg gtaacaagga cccacggggc caaaagccac gcccacacgg
      661 gcccgtcatg tgtgcaaccc cagcacggcg actttactgc gaaacccact ttaaagtgac
      721 attgaaactg gtacccacac actggtgaca ggctaaggat gcccttcagg taccccgagg
      781 taacacgcga cactcgggat ctgagaaggg gactggggct tctataaaag cgctcggttt
      841 aaaaagcttc tatgcctgaa taggtgaccg gaggtcggca cctttccttt gcaattactg
      901 accctatgaa tacactgact gtttgacaat taatcatcgg catagtatat cggcatagta
      961 taatacgact cactatagga gggccaccat gaagaaacct gaactgacag caacttctgt
     1021 tgagaa
//
