LOCUS       sequence-107277-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA with 1x MS2 for yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC545
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..40)
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     misc_RNA        complement(43..61)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(66..141)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(162..430)
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     primer_bind     complement(455..474)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(456..474)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(481..498)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(481..497)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(490..512)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gctggtaccg aactctgcag agacataaaa aacaaaaaaa gcacatgggt gatcctcatg
       61 tgcgcgcacc gactcggtgc cactttttca agttgataac ggactagcct tattttaact
      121 tgctatttct agctctaaaa ctatcagtga tagagaaaag tgatcattta tctttcactg
      181 cggagaagtt tcgaacgccg aaacatgcgc accaactttc acttctacag cgtttgacca
      241 aaatcttttg aacagaacat tgtagggtgt gaaaaaatgc gcacctttac cgctagccca
      301 agagggcact acaaaatcta gagttgtact tcaaacgtac atgtaatcac cttgtatata
      361 ctcgaaagaa aacatcaagt ttctgtataa atatgagtga aagcataatc atacattatc
      421 ttttcaaaga gaattccgag tgagctccaa ttcgccctat agtgagtcgt attacaattc
      481 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      541 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      601 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
      661 agcgcggc
//
