LOCUS       sequence-107280-         595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA with 2x MS2 for yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC583
FEATURES             Location/Qualifiers
     source          1..595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..40)
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     misc_RNA        complement(54..72)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(83..101)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(108..183)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(204..472)
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     primer_bind     complement(497..516)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(498..516)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(523..540)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(523..539)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(532..554)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gctggtaccg aactctgcag agacataaaa aacaaaaaaa gggaacacga gcgacatggg
       61 tgatcctcat gtcgctcgtg gcacatgggt gatcctcatg tgctcccgca ccgactcggt
      121 gccacttttt caagttgata acggactagc cttattttaa cttgctattt ctagctctaa
      181 aactatcagt gatagagaaa agtgatcatt tatctttcac tgcggagaag tttcgaacgc
      241 cgaaacatgc gcaccaactt tcacttctac agcgtttgac caaaatcttt tgaacagaac
      301 attgtagggt gtgaaaaaat gcgcaccttt accgctagcc caagagggca ctacaaaatc
      361 tagagttgta cttcaaacgt acatgtaatc accttgtata tactcgaaag aaaacatcaa
      421 gtttctgtat aaatatgagt gaaagcataa tcatacatta tcttttcaaa gagaattccg
      481 agtgagctcc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca
      541 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacat
//
