LOCUS       sequence-107281-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human immunoglobulin epsilon antibody expression vector (kappa light
            chain) without variable regions. Enables Colony-PCR screening of 
            false-positive (PCR vector template) colonies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, 
            Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
  TITLE     A tool kit for rapid cloning and expression of recombinant 
            antibodies.
  JOURNAL   Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885.
  PUBMED    25073855
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-07-30"; volume: "4:5885. doi"; pages: " 
            10.1038/srep05885"
COMMENT     Sequence Label: pVITRO1-dV-IgE/kappa
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcatttaccg ggatttacag acaccgctcg ctggacggtc tgtgaggggc tcgctgcctc
       61 atggactgca cggcagatga actcatcttt ctgctcccat tcggccctgg tcacctccag
      121 gcggctgaag acgaagaagc cggagccctt ggtcttgcgg ggctgcgtcg tgctgtgccg
      181 ggcgtccggg agctgcacct cgttgtgcag ccactgcacc gagatgtcct caggcatgaa
      241 gttctggatc aggcaggcga gggtgcgctt gtcccggctc cccggccact ccggcgtcgc
      301 aaacgcatag acttccgggg cagcacgcgg gccgctggtc ttggtcgtgg accgcatgag
      361 ggccctgggc aggtgggggt gggtcaccct gcactggtag gtctccccct cgatccagtc
      421 tcgggtgccc accggcaggg tggacgtgac ggttaacgtg ccattgcgct gcttctcctc
      481 ctttctggtg gagtggttca caggcttccc actggcccgg gaccaggtca ggttcacggt
      541 ccccttgctg ggtgccaggt ccaccaccag acaggtgatc gtgggcgact tgcggatgaa
      601 caggtcgaac gggctgggcc ggcttaggta ggcgctcacc cctctcgggt tggaatctgc
      661 acacttcttg gtgctgtcct caaaggtgtg accttgatag gtgacctggc aggtgtaggt
      721 gcggtctgac agccagtgct tctggctgag ggtgagctcg ctttgtgtgg aggccagctc
      781 accctcctgc gtggtagagg cggtggacaa gtccacgtcc atgacctgcc cgtcctccag
      841 ccaggtgatg ttga
//
