LOCUS       sequence-107283-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.HO6
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      647..704
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(728..745)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
       61 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggtctcattt tactagtgca
      121 tgctttaaag tatagataga attgattgct gcttatgagg atatggattt agagctaata
      181 gttgtaatag aaataaaagc catttagaat aggaattgaa tacaatttta tgagctttaa
      241 atatttaatg atgaatagtg cgtttagaac gcttcatcat gcttcaacaa gcttctgcaa
      301 acctcatttg agtagcaata gctgtttact tttttccgcg actttattcg aagttatggt
      361 gaacttttcg tggtttcaca cctaataacg cccagcggta gtacaattca aagtagtagg
      421 taccaatggt agtactagtg ggtgatagta gcaaatttct ctatgaaaat gaattgttgc
      481 tctgcaatga tgttaatgtg gacatgacat gaattttcgc gatatgttga tgtaaataat
      541 aacatgattt tcgtggatca tttcgtaaac cataggttta tttcgtgaat tactaagaga
      601 aaggcataga aacaggactt gcggatggga tacgcggccg ctgcagtccg gcaaaaaagg
      661 gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc
      721 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      781 ctcactcaaa ggcggtaata cggttatcca cagaatcagg
//
