LOCUS       sequence-107292-       15080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and
            3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15080)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 15080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW6.01
FEATURES             Location/Qualifiers
     source          1..15080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(26..481)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(163..184)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     375..394
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     608..630
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     622..639
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     623..639
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     649..668
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        649..667
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     692..708
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     693..709
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2666..2677)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(7599..7627)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     8375..8395
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     rep_origin      complement(13330..13918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(14089..14880,14881..14949))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14712..14731
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14950..15054)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaattctatg aaaatgtcta aagaaaaatt gtaaacgtta atattttgtt aaaattcgcg
       61 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
      121 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
      181 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
      241 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
      301 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
      361 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
      421 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
      481 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      541 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      601 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca
      661 ctatagggcg aattgggtac cgggcccccc ctcgaggtcg acggtatcga taagcttcag
      721 taaaagaagt agaattttat agtttttttt ctgtttgaaa aattctcccc atcaatgttc
      781 tttcaaataa atacatcact aatgcaaagt attctataac ctcatatcta aattcttcaa
      841 aatcttaaca tatcttatca ttgctttaag tcaaacgtaa cattaaaaaa aatgttttgg
      901 aaaatgtgtc aagtctctca aaattcagtt ttttaaacca ctcctatagt cctatagtcc
      961 tatagttacc catgaaatcc ttatatatta ctgtaaaatg tttcaaaaac cattggcaaa
     1021 ttgccagaac tgaaaatttc cggcaaattg gggaaccggc aaattgccaa tttgctgaat
     1081 ttgccggaaa aacggtaatt gccgaaagtt tttgacacga aaatggcaaa ttgtggtttt
     1141 aaaatttttt tttttggaaa tttcagaatt tcaattttaa tcggcaaaac tgtaggcatc
     1201 ctaagaatgt tcctacatct attttgaaaa gtaagcgaat tctatgaaaa tgtctaaaga
     1261 aaatggggaa acaatttcaa aaaggcacag tttcaagtgt ttccgaatta tactaaatcc
     1321 ctctaaaaac ttccggcaaa ttgatatccg taaaagagca aatccgcatt tttgccgaaa
     1381 attaaaattt ccgacaaatc ggcaaaccgg caatttggcg aaatttgccg gaaggcaatt
     1441 gccgcccacc cctgttccag aggttcaaac tggtagcaaa gctcaaaatt tctcaaattc
     1501 tccaattttt ttttgaattt tggcagtgta ccaaaatgac attcagtcat attggtttat
     1561 tatagattta tttagataaa atcctaaatg attctacctt taaagatgcc cactttaaaa
     1621 gtaatgactc aaacttcaaa ttgctctaag attctattga attaccatct tttcctctca
     1681 ttttctctca ctgtctattt catcacaaat tcatccctct ctccctctct ttctctctcc
     1741 ctctctctct ttctctttgc tcatcatctg tcattttgtc cgttcctctc tctgcgccct
     1801 cagcgttccc cacactctct cgcttctctt ttcctagacg tcttcttttt tcatcttctt
     1861 cagccctttt tcgccatttt ccatctctgt caatcattac ggacgacccc cattaatttt
     1921 tggatgtgct ctgcgagcaa gagcacacgg aactcattta ttttttgttg gaatttcttt
     1981 ttcattttag ttagttaaaa ggttattcag tgcaaaaatt gattttttgc tcttttggta
     2041 cattagaggg gtttgaaagt tttccataaa aagggtttcc ggagtaaaaa atctaaaaat
     2101 ttttacaaac atttccaaaa attttgggaa catatctttt caaaaactca aaaaaaaacg
     2161 caaacatcat aaatttaatc aattaattgg atgcgttcaa atattttaaa aatatttttg
     2221 aattgcatat ttctttactc ataaaaaaat tattaatgtt taagtaaaaa aaatatttca
     2281 ggattttaaa caaaaactgt aaatcccata aaacagtgtt attttttagt tatttttagt
     2341 aattatttgt gaattaaaaa cacatttttt ttcaaaatat tccttgaata aataaaattg
     2401 aaagtgaatt caaaatattt caccaatttt acaaaatttg aaaatttgaa aaaatatttt
     2461 ctaccaaatt gtgctcaaaa aatattatta agttcccagg aaatttatga tgaaaacttg
     2521 gaatattttt ggtaaaattt tctcaaaaaa ttgaaagttg tcaaaaacac actaacacta
     2581 tacctcgcgg catagaaaaa actggtggcc gaatttttta aaccaattta aattaaatat
     2641 atttttgcac tttgtacaaa aaaatttgaa gttcccagga aattcatacg gaaatatttt
     2701 ccagaacttt tataattttt attttaattc tcaaaattta caataaaaac ttggaaacct
     2761 gaaatttgct taaagaataa ccctatcccc cacggcctag aaaatactgg tggccgaatt
     2821 ttttccgcag ccacaccacc tctaatctcc agagcactct ccaaaattcc cataatcccc
     2881 aaaattttca gctacaacag catatatgaa ggcagagcaa caacaacaat cgatcgcacc
     2941 cggctcggca accttcccgt ctcaggtgag gaagacgcag agaaatgaga gagagagcgt
     3001 gtttatgcac gcagtatact aactcctgat atccaatttc atatatcgat atgcttgctt
     3061 tcttttcttc tacgttgaaa ttgtttgaac ttttctcaaa aaacgcggat tttagagaga
     3121 cttagagttg ctccaaataa atttaatttg caattttgca cttactatta gaatctactt
     3181 ttatagcgct aaaatgaccg aataacacag tagattttaa aagaaaattt taaaaattat
     3241 ttttttgtgc agtcaaattt cgctagtttt tgccactcat ttttgaacat ttgtatatgt
     3301 gcctttcggt ggttcaatca ggggtggcac gtggtgtcag agtgttccat ttcggtttga
     3361 tctacgttga tctacaaaaa atgcgggaga agagacgcag agttctcaac tgatttcaca
     3421 tggttatgaa ccaggggtgt tcggcaaatt tgccgtttgc cgagctgggc aaattttgag
     3481 atttgccgca cacccctttt atgaacgtgc tgacgtcaca tctttttgga caaagaattc
     3541 ccgcattttt tgtatatcaa accgtgatgg gacagcctgg caccacgtgg ggttggcggc
     3601 aaatgatttt ttccgacaaa tcggcaaatt ggcaaattgc cggaatttaa agtttctggc
     3661 aaattgttga tttgccgaaa aaaaatttca aaaaggcaca gttttaagtg tttccgtctt
     3721 ataaaatatt tctctaaaaa tttccggaaa acggaaaatt gccggaaata caaagtttcg
     3781 tcaaatcggc aaacctgcaa tttgccgaac ggcaactgcc gccaacacct ggttcccaaa
     3841 aaagcgtgca ctgtttgaat attaaaaaaa aaattcacac aaaaataata aattgaaata
     3901 ggctaaattc acatctatgt acaaaaattg catgccagca ccggtcaatt ttctcaatta
     3961 ctttttgtct atttttgccg aacataattt gctgtttcct tggcagaata cagaaaactc
     4021 tttttttttt cagatgcccc gacccccacc ggtaaccgaa caggctataa ccaccgaggc
     4081 ggagcttctc gcgaaaaatc aaattacacc aaatgatgtg ctagctcttc caggaatcac
     4141 tcaaggtaag tgaatatttg ctctattgat aaacgcgccg tgtactccac gtggacaaac
     4201 atatcaaatt ttcaggattc ctatgctccc catcggcaaa cgtctataac attgagttca
     4261 ccaaattcca aatccgtgac ctcgacacgg aacacgtgct cttcgaaatt gcaaaaccgg
     4321 aaaatgagac ggaagagaat ctgcaggcgc aagccgaatc ggcaagatat gtccgatatc
     4381 gatttgcgcc gaattttctg aaattaaaga cggtcggcgc gacggtcgag ttcaaggtcg
     4441 gcgatgtgcc gatcacacat tttcgaatga ttgaacggca ctttttcaag gatcgtttat
     4501 tgaaatgctt tgattttgaa tttggattct gtatgccaaa ttcgagaaac aactgtgaac
     4561 atatctatga atttccacaa ctttcacaac aactcagtga gtttgaagcg ggaaaaatta
     4621 tttgagctca ttttgtcaat agagcgaatt ttctaggttt ttaaaaataa aataaatatt
     4681 gacaaaaagc tgccctacag tagttcaaaa tgtgaaaact tggaggaaat tcgagttttc
     4741 tctgagaagt ttttggcggg taattcaaat tttctgagaa aaaaattagc cgcgagaatt
     4801 caaattttct gagaaaatta tttgacggga aattcaaaat ttctggaaat gtattgaaca
     4861 tttgagaaat tttgttaaaa actacattaa accgataaaa aagttcgcaa atttttttta
     4921 agtgctcatg gtgcatcgaa gagaagaatt cttgccaaaa ctagcccatg aaattaaaaa
     4981 ttatcgcgga aaaaaactga ctaattggaa agtaaaatga aatttgcaaa aaaaaaatat
     5041 gtttttttgt tgtaaaatta aaaaaaaaac tgtatatttt cagtggacga catgatcaat
     5101 aatccaaacg agacccgctc cgatagcttc tattttgtgg agaataagct cgtaatgcac
     5161 aataaggccg actactcgta tgatgcataa tttcccgccg atggaataaa actaataatt
     5221 tttctcgcaa tttatagaaa tcatgtgatt ttcctaccgc taaatttaaa atttcctaat
     5281 ttttcactat atttctcaca aaatgcattc cgaaaactct catttttcag cttttcaata
     5341 caaatcccaa taaatgattt ttcaaatata atgttctcaa ttgatatgtt ttatttgaat
     5401 gttggaaaaa accataaaat taaaaaaaac ttgaattatt caaaaataat tcggcccttt
     5461 tacaaaaaat tatcataaaa attaaatttg gaaaatctgt gatttgtcgc tttgccggaa
     5521 gtttccaatt ccggcaacta accgatttgc cgattagcta gaaatttcaa ttccgacaat
     5581 gtgccgcttt gccgatttgc cggaaatttc caattccggc aattaacgga cttgccgatt
     5641 agctagaaat ttaactttag ataaaacttc tcaacaaaat caaattggaa agtcagcgat
     5701 ttgccgcttt gccggaagtt ttcagttccg gcatctaacc gatttgccga tttgccaaaa
     5761 atgtcaattc cgaaaaactg ccgatttgcc gctttgtcgg aaatttcaat tccgacaaat
     5821 tgccgatttg ccgatttgcc gaacattcca attccgacca tttgtcgatt tgccggaagt
     5881 taccaattcc ggcaactaac ggatttgccg atttgccgga aaaaaattgt ttgtcgccaa
     5941 ccctgattta aatgactaat catgttgcaa aagataactg cttgcatcaa aaatccgatc
     6001 attggcattc ccaacatctt cttcatccaa aacaccaccc ttttcttcca ctaatcatga
     6061 attcccgacc aattgttatc agtaggagaa gcctcctttt atctcattat cagcaattgc
     6121 cttcttcttc ttcttctttg aataccattt gaatggagat atcatttaca taaatctgcc
     6181 gccgagattg tgaagcagga ggaaaagctg aagatactga tattatatgt atatttcatt
     6241 attctttttc cacaaaattt tccgacattt aattccactt cactttcatc gtctcttttt
     6301 cccccattgt cgcccccact catttgtctt cttcttcaaa tacgggtcaa tcactttccg
     6361 ttttttattc attttttgtt atgttcggct taacaaattt gaagtattgt taaaattctc
     6421 tagagcggcc gccaccgcgg accaaatggt aactcaaata tgtaatcgaa aaaaaaaaac
     6481 actgagcaga ctaacggagc aggcgagcgt ctaaatggta taatcgatac atagaaagtg
     6541 gttataaatc ctccaagtgt gtgacatcat acattgtgca caatgtagca gtagctatag
     6601 tagcaggaaa ttagtaacat tagatctatc ctgtttgaaa gcatcaatgt cgatgagttg
     6661 ttttttaaat taaaaaacca tcgagaataa tttgaaaaat aaaatatgaa taatctgaag
     6721 tcccaaggaa taccaaattt ctacttgaag attctcagtt tttgtaactt tttagaagtc
     6781 ttcgatcaaa aaaaatcctt tgaatagaca ttttttcata tttttatgaa ttaataatgt
     6841 taagaatcct tattagtttg ggatatccaa acatttttta aaatttatgt taaaaaacaa
     6901 aagatttcaa tgttctatat aaagcagttt ttattacttt ccaggtactt agtattccta
     6961 tattaaaata attttaaaaa atttcaaaac tctgcacaaa tccgtttttg gagctatttt
     7021 aacgaccctt tacatctcgt tttcaggaat tcaggattat gttccgccgt ttcttttaaa
     7081 aagtttgaga taaaaaatta ttttagtttt gatttttcgc ttacaggttt tctaattaac
     7141 atctccttta ctgcctatta tcctgtttct ttttataaat ttattcttct catttattat
     7201 tcagtgatta tccaatattt tagagaatta actatcaatc caattgttcc tcaaaactcc
     7261 atagtcttgg atagtcttga aaactgatcg ataccatgca aacggtgtgt gttgttttgt
     7321 tgattccctg ccgcatttga actatttttt caccgcgcgc acgcatcgcc cctgtccgtc
     7381 tcgccacaca ctatttccgc gcgctctttc tctacctctc acactctctc aatctcccta
     7441 actcaagtgt tcattcttgt tgcgtggtcc caccggtttg cattttttca tctctgtctt
     7501 tctttttcgt tgtatttttt taaatttcat ttatttcaat atttcattaa gttttgattt
     7561 ttgcaggtat gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     7621 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     7681 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggg
     7741 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag ccaacacttg
     7801 tcactacttt ctgttatggt gttcaatgct tctcgagata cccagatcat atgaaacggc
     7861 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
     7921 aagatgacgg gaactacaag acacgtaagt ttaaacagtt cggtactaac taaccataca
     7981 tatttaaatt ttcaggtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     8041 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     8101 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagttg
     8161 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag aacttcaaaa
     8221 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     8281 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     8341 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     8401 ctgggattac acatggcatg gacgaactat acaaaatgag ttcctcaaaa ccttacccaa
     8461 gcggtctgcc aaactcgaga agaaaaagag gaggtgagag ttttttgcat caatttttca
     8521 ttcaatttgt aaattcaggt cgccgctcaa gtagtcgtag caatcaggaa agtgcatcca
     8581 acaacatgga acaccaaatc actcttgacg aattgttcaa tccgatcgca aaacaagaca
     8641 gtgctcagtc aacaagccgt gaatatggtg caaaatcggg gatatctcat cacggatccg
     8701 tatcattcaa cgggaataca ttcatgaatg gccaacagct gaatcactcg atgactcgac
     8761 atggtcgagt attcaatcag tcgatgcatg cagctcaagg aaacggcagc aacgccttta
     8821 acagtattcc tcctacggcc cccgtcttct ctgcagactt ccgccgcaac ttgcaaacac
     8881 gcaatagctc cagctggtat gaacgaagat tcccagtatc cacagatcag gacgacgttc
     8941 agcaatcaaa cacaagacgt agtagaagtc gtcagaatgg acaacatggc ctaagcttct
     9001 cggatggttc gaacaattat ggtcacgctg ggaataagtc attcagtgtg agttccgttc
     9061 ccgttgggtt ccagaagcaa gaaaataatt ctaagaaact gcgacaaacc aatgttcacc
     9121 agcaatgtct tggaaataaa agcttcaacg ctcaagctgg agttcatgga cacgctttta
     9181 aaaaaggcca taaggacaat aaaaatgcat ctggaaagga ggttatcaat tctagtcttg
     9241 tccaaaagca cgatgctatt aagtcgcgga atttgaacca gagcttctct ggatttccta
     9301 cacatgaaac ctcatcgatg aaaaatcaac aacagaaatc aagaaacgac agaaaaaaat
     9361 cacgtggtag cagcaacttc caggatcgta cttacttcaa cacaaatgac gatgaattaa
     9421 ctgacgatgt gttcatagac gattccatgg atgctgcccg tggtcggaga tctcgatcag
     9481 tcactaagaa acttcaacaa tcgacttatt caaagcagaa tgcaggcagt aaacaattga
     9541 ctgaaaaatg caaatcttct gaagaagcgg ctaaacgtaa cctggtatcc aatgtattca
     9601 gcaaggacgg cactgaactc tccattgaac agttattgga aattgtcagc atgaagatcg
     9661 gacaacagat tcacttgcct tccagctctc atggagaatg cagcaatttg aatcggacat
     9721 taccagcaag tgatctaaat tgctcaattg gtgaagactt tgactccagt tttgtggatg
     9781 ctaacaacca aaccctacca gtgagtcttc ccaagaaaac ttcactgtcg atcaagcgcc
     9841 gtggaagttc tcgtagtgcc tcccgtctgg ccagtcttga tgtgacactg gaaactgttg
     9901 aagaagacga ggaacccacg ccaagtccac agccgagttc tcctccaaag atctcaagaa
     9961 gaaagtggac aggaactttt gatgccaacg ttgaggaaat gagacgttta cttcatgggg
    10021 accctgagat gccgaaatct gcaaatcgtg cttcctcaag gtatgtagtg aaatagtaaa
    10081 atctttagta aaaatttaaa tttcagcaag gatcagatca atcggaacaa tgtggatgta
    10141 aagagaacac cttcctcgtc aatcatccca actccaaagg ctttgatcgg agaaagatgt
    10201 ctgacatcat ccagcaagtt agttttacac ttggagcttc ttatttcatt atatttttta
    10261 caggtcttca aagttgaaca agtcacttgg cgtggttgat tctaaagcta caaaatcacc
    10321 aatgtattcc gtcacagttt ctggaaaaga aacagcttca ggaaagcgaa ttgctcaaaa
    10381 gttgactcca aaggttgtgg cgctggaaag gtatgacttt acaaaaaacg aacaacattt
    10441 ccatattttc ttttccagct cttacattac cggaattcca gtttccacgg attgtaatgg
    10501 ttgcccaact ccgaagagaa gtggaatcaa ttgtgaaatt cgtgcagctg aagtatacaa
    10561 tcaagctggg aaatggccct tcgaaatcac caggtaaggt ccaagttaat aaatccgcta
    10621 tcgatcaagc atatggaaaa taatgtacaa gctgaaaaga ttgagctcaa aacttgattc
    10681 aaatcctgta aaattatttt ctgagtacag taaatttctt tcagtgatcc ggctccactt
    10741 ccttgtgaat cagccgatcg gatcgaatat ccttctcaag attgcactca agatccagct
    10801 tcaacctcgc caccacctcg catttctgaa agcttgacag cattcctgga agctcagcaa
    10861 gactttaacg actacattga tactaactac aaagaaaaga cacaactcct gaaagtgaat
    10921 ttgaatattc acgggatgtc acctgaaaga tggctgtact tgaattattt ttgcaccgag
    10981 acaattccaa gattggatgg tccttatgcc gatggtaaga aagataagac atatttaatc
    11041 ttgaaaactt taattttttc agatccaaga gttccacctg tgcgaaatat gttcagaaaa
    11101 tggttcctcc gttttgctga agcctgctta ggaaatcctc accagcttgc agttatgcaa
    11161 gagattgctg ccacgtttgt tcaggctcgg ctggacgata cgagcagctc cacagactcc
    11221 accaatatgc tctacatgtt gtggaaggag tgtatcggac agaagaatat catcgcaatt
    11281 gctgatgcgt gtttacttgc acatcttcgg aaatcggatc ctatcaaata cttgaatgta
    11341 aaacgggatt ggctggaatc tatttttgac ccacccagag atcaatgatt gtaccaattt
    11401 atatctatta cttgtagact atattgtatg ttattcttgt tcattaaccc tttatccact
    11461 cttgttatat ttcaactcgc ttctccttgt gaatttgtat tatgtctacc cagatacttg
    11521 tagagggtag tattatattg ttgtctggat gattgataaa gaattgccga ttctttaaga
    11581 tttggagtgg aactagaata aaagaaaagt tcgaaataga tttaagaaaa gatgattggt
    11641 aactgatttg aaaatataca tatcttctca tagatctttt ttgtgaaatt taaatactgt
    11701 cattaggcaa aatagtagtc acggattcaa ttttaaaaca gatgcttcag atttgtttgg
    11761 aaaagttccg ttttttaata cagggtgggc cataattatg cgcactactt tgaagcttca
    11821 taaaaccaaa acaaaaaaca cattaaacaa ataatcatat aggttttgtt ctccgagaaa
    11881 aactaaataa gataccattt ttgaattcga aaatatcacc gtccgcattg ctacaggcct
    11941 tcacaagttt cggaaacgca ccaacgatgg cggacaagta ggcacgcttc agggtttttc
    12001 atgtttttct cggaggactc attagttatc aagtaaaatt taaagttcaa agaatgctct
    12061 tgattcagag atgcttcaca caaaaaatct aaaagattga tattgttagg tgacagtggc
    12121 agctgattga gtatcgagta ctgtgaaatt cagtcaaggc ctaaacgaag cttgtgcgcg
    12181 gaagcactga catgctaaga aaatcatatt tgtgttttta gttattattt ttacccaagg
    12241 tttcatattt tcttacgaat ttgctcatag atgattttgc ggatcggtgt gtctcgtgga
    12301 acagaaaaaa gtgatgcgaa ctaatgggac ggtctttaaa acacacacta ttatccatca
    12361 ttatatccat cagtgttggc gcataaccac ttttgctgat tcaaaacata cgtaactggt
    12421 tcacagctac ttaaacttca tattaatgct gaaaatcaaa attttttgga atattggccg
    12481 gtggtacagg ctctccgcca aaacaccact ttggtagagg ggtgcttcag attgtcgtgg
    12541 ttgtggagag tgcatcttgt tgagaaacat attaaatttt atgtcaaatg gaagataatc
    12601 atcccggcaa ccagaataaa ttaaaaaatt tgagacatga cgtggcagtc gcgtaaaaaa
    12661 ctacagtaaa taggctagaa acgagtgcgc ataattatgg tccaccctgt aaagtaaaaa
    12721 atcatgtgaa acatctggta gcaaacaaat agttgaaaag catactccag gttgacaatt
    12781 ttcacaatca gatcttgtac atacgagcat taaactatta gtttcagttg tgctgtgaaa
    12841 actcaatttt cactacaaaa aagaacacga tttcaaaata tattcctaac tctaatgcaa
    12901 gaagtagcac tcaatttgaa atctcacaaa ttttgaccaa agatcttctc gaaattactt
    12961 gcaaccacac caactgacaa acactgagaa caaacactgg agagcacctg ttgttattcc
    13021 ttgctatttc ttcgtttgtt gtaagctcgg ggagttttac ctccatactt ctttactttt
    13081 tttctctaaa tgtttttttt caagattaaa aataaccagc aacctaataa aattaattcg
    13141 actgttattt tagttgaaat ttgtgtcttc acacaagtcc gaactcggag acaaaatttt
    13201 tgaagtttca taatcttttc acagagaaaa taaaactatc aaaatgcaaa cgtgaacaag
    13261 gcggccgcac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    13321 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    13381 tcagaggtgg cgaaacccga caggactata aagataccag gcgttccccc ctggaagctc
    13441 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    13501 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
    13561 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    13621 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    13681 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    13741 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    13801 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    13861 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    13921 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    13981 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    14041 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    14101 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    14161 gcccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    14221 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    14281 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    14341 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    14401 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    14461 ccaacgatca aggcgagtta catgatcccc catgttgtga aaaaaagcgg ttagctcctt
    14521 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tgcttatggc
    14581 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    14641 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    14701 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    14761 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    14821 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    14881 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    14941 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    15001 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    15061 tccccgaaaa gtgccacctg
//
