LOCUS       sequence-107294-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Scaffidi P, Misteli T
  TITLE     Lamin A-dependent misregulation of adult stem cells associated with 
            accelerated ageing.
  JOURNAL   Nat Cell Biol. 2008 Apr;10(4):452-9. doi: 10.1038/ncb1708. Epub 2008
            Mar 2.
  PUBMED    18311132
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1708"; 
            journalName: "Nat Cell Biol"; date: "2008-04"; volume: "10"; issue: 
            "4"; pages: "452-9"
COMMENT     Sequence Label: pBABE-puro-GFP-progerin
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(42..44,45..47,48..758)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(42..44,45..47,48..758)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(87..108)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(348..367)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     695..716
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cggatcccag tgtggtggta cgtacgctac cggtcgccac catggtgagc aagggcgagg
       61 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      121 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      181 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      241 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      301 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      361 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      421 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      481 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
      541 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      601 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
      661 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      721 ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga tctcgagctc
      781 aagcttcgaa ttctgcagtc gacggtaccg cgggcagcgc tgccaacctg ccggccatgg
      841 agaccccgtc ccagcggcgc gccacccgca gcggggcgca ggccagctcc actccgctgt
      901 cgcccacccg catcacccgg ctgcaggaga aggaggacct gcaggagctc aatgatcgct
      961 tggcggtcta catcgac
//
