LOCUS       sequence-107300-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre recombinase-dependent expression of DsRed, i.e. DsRed is only 
            expressed in the presence of Cre due to a transcriptional stop in 
            between the loxP sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Controlled expression of transgenes introduced by in vivo 
            electroporation.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 
            10.1073/pnas.0610155104
  PUBMED    17209010
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2007-01-16- 16"; 
            volume: "104"; issue: "3"; pages: "1027-32"
COMMENT     Sequence Label: pCALNL-DsRed
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(72..749)
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(134..157)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     549..569
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     regulatory      746..755
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    827..860
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(898..917)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     952..971
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tgtgagccag ggcattggcc acaccagcca ccaccttctg ataggcagcc tgcacctgag
       61 gagtgcggcc gctacaggaa caggtggtgg cggccctcgg tgcgctcgta ctgctccacg
      121 atggtgtagt cctcgttgtg ggaggtgatg tccagcttgg cgtccacgta gtagtagccg
      181 ggcagctgca cgggcttctt ggccatgtag atggacttga actccaccag gtagtggccg
      241 ccgtccttca gcttcagggc cttgtgggtc tcgcccttca gcacgccgtc gcgggggtac
      301 aggcgctcgg tggaggcctc ccagcccatg gtcttcttct gcatcacggg gccgtcggag
      361 gggaagttca cgccgatgaa cttcaccttg tagatgaagc agccgtcctg cagggaggag
      421 tcctgggtca cggtcgccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag
      481 ccctcgggga aggacagctt cttgtagtcg gggatgtcgg cggggtgctt cacgtacacc
      541 ttggagccgt actggaactg gggggacagg atgtcccagg cgaagggcag ggggccgccc
      601 ttggtcacct tcagcttcac ggtgttgtgg ccctcgtagg ggcggccctc gccctcgccc
      661 tcgatctcga actcgtggcc gttcacggtg ccctccatgc gcaccttgaa gcgcatgaac
      721 tcggtgatga cgttctcgga ggaggccatg gtggcgaccg gtggatcccg ggcccgcggt
      781 accgtcgact gcagaattca atttaaatcg taccgagcga ctcgagataa cttcgtataa
      841 tgtatgctat acgaagttat acgcgttcgg attgatccag acatgataag atacattgat
      901 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      961 gatgctattg ctttatttgt aaccattata agctgcaa
//
