LOCUS       sequence-107305-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB2 (small HSP) tagged with V5  in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-V5-hspB2
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(614..655)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(758..782)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    792..810
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 actcgatttt ggcgcgtttt gctgggtgct gggtctgtgg caatcagggc tcaactatgg
       61 ctgcctcctc ctcttcctct ggatcaggag gcgcagggag cagggagatg tagacctcat
      121 tgacctctgt gtccaaatgt cggccacccc gaggtgcttc caggtttaag atgccatcat
      181 gggagagagc agctcggact cgccaggggt cgacatcagc aggcaggaca taggtgcggc
      241 agaactctcg ggacacgaag ccgtggcggt ccaggcgctg ggggtgccgg gcagacacct
      301 ccagcaggtt atccacagtc ctcacagtca cctcgtctgg ggtaaagtgg ctcacatcca
      361 gaaatgcctg gaacttgccc tcactgagcc taagctcgga ggcccctgcc ctgctgccct
      421 ccccagctgg ggcggcccga ggccggacat agtagccatg gtagagtgtg ggggtcagga
      481 tctcttctgg caggaggcct tctccgaagc gctgctcacc caggcggctc gggttggcaa
      541 attcgtactc ggcggtggcc gggtgggcat gtggcactga gcggcccgac atggttctcg
      601 aggttttgga tcccgtagaa tcgagaccga ggagagggtt agggataggc ttacccatgg
      661 tgaattcaag cttaagttta aacgctagag tccggaggct ggatcggtcc cggtgtcttc
      721 tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag
      781 ctcgtcgacg atctctatca ctgataggga gatctctatc actgatagg
//
