LOCUS       sequence-107306-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB4 (small HSP) tagged with V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-V5-hspB4
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(577..618)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(721..745)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    755..773
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    776..794
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(796..847)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(825..845)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 actcgatttt ggcgcgtccg aggcaatgcc tgcttaggac gagggagccg aggtgggctt
       61 ctcctcccgc gacacgggga tggctcgctc ggcgtgggtg gcatccaggc cagtctggat
      121 cttggggcca cagaaggtca gcatgccatc ggcagacagg gagcaagaga gggccgactg
      181 gtccacgttg gacggcaggc ggtagcggcg gtggaactca cgggaaatgt agccgtggtc
      241 gtcctggcgc tcgttgtgct ttccgtggat ctccacaaag tcgtcctgca ccttcacggt
      301 gaggtcctcc ggggagaagt gcttcacatc gaggaagatg acgaacttgt cccggtcgga
      361 tcgaacctca gagatgccgg agtccagcac ggtgcggaag agggactggc ggtagtaggg
      421 gctgatggtg gacgacagga agggcagcag gtcatactca aaaaggccct cgccgaaaaa
      481 ctggtcgaac agccggctgg ggtagaaggg ccccagggtg cgcttgaacc aggggtgctg
      541 gatggtcacg tccatggttc tcgaggtttt ggatcccgta gaatcgagac cgaggagagg
      601 gttagggata ggcttaccca tggtgaattc aagcttaagt ttaaacgcta gagtccggag
      661 gctggatcgg tcccggtgtc ttctatggag gtcaaaacag cgtggatggc gtctccaggc
      721 gatctgacgg ttcactaaac gagctcgtcg acgatctcta tcactgatag ggagatctct
      781 atcactgata gggagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
      841 ttgcgtcaat gg
//
