LOCUS       sequence-107307-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB6 (small HSP) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-hspB6
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(669..693)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    703..721
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    724..742
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(744..843)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(773..793)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 actcgatttt ggcgcgtgag cctgaggagg ctcccggggt gcgggcgcgg cccagccccc
       61 tcctacttgg ctgcggctgg cggtggggcc tgggccgacg ctggtgcggc ctggatggac
      121 aggacgccct cgggggacag cgcggacgtc acggcagccg gatccacgcc aggcggcagg
      181 cggtagcgac ggtggaactc gcgcgcgacg aatccgtgct catccgggcg ctcctcgtgg
      241 cgcgcgtgca cctccacgtg ttcgcccacc accttgacag caatttcctc cggcgagaag
      301 tgcttcacgt ctagcagcac cgaaaagtgg ccggggtccg tcggcacctg ggcgacgggc
      361 agcgccacgc tgggtgcgcg caggtagtag ggggcgagcg tggtggggca gagcgcagcc
      421 agctcggcct ccagcagccc ctcgccgaag cgctggtcaa agaggcgtcc gggcgccgaa
      481 agtccgggca acggggccga ggcgcggcgc agccaagacg gctgcacagg cacagggatc
      541 tccatggttc tcgaggtttt ggatccgagc tcggtaccaa gcttaagttt aaacgctaga
      601 gtccggaggc tggatcggtc ccggtgtctt ctatggaggt caaaacagcg tggatggcgt
      661 ctccaggcga tctgacggtt cactaaacga gctcgtcgac gatctctatc actgataggg
      721 agatctctat cactgatagg gagagctctg cttatataga cctcccaccg tacacgccta
      781 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt
      841 tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag
      901 tcaaaccgct atccacgccc attgatgtac tgccaa
//
