LOCUS       sequence-107309-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hRPN13 (285-386) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pDEST15.1-hRPN13 (285-386)
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..53
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(475..495)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(516..540)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(594..616)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 tagcagccgg atcccggggt cgaatcaaac cactttgtac aagaaagctg ggtcggcgcg
       61 cccacccttc gaggatccta ttatcagctc caggctcatg tcctcctctt cgtccttctt
      121 gtccttcgtg tcgccctctt tctgctcggg ttaagcttac ttggcgttgt tctgcatggc
      181 tttggcaaac gcttccacat cgcccttgtt ggcggcctcc acagcctctg caggcagacc
      241 gaactggcac atgagggggc ccagctgccc cgaggccaag gctgcgctga acatgcccag
      301 ggcctgctgg aactggggcg aggtcagggt attctggatc tcatccgcgg tctgcggcag
      361 cgactcccca gatggcaagt agggaagcag gcgctcctgg acatccgcgt tggcgaggat
      421 gggagccatt atctccggcg tcagcacact ggccaggtcc acttgggtcc aggagccctg
      481 aaaatacagg ttttcctcga gtacggcggc cgcggagcct gcttttttgt acaaacttgt
      541 ttgattcgac catggacgcg gaaccagatc cgattttgga ggatggtcgc caccaccaaa
      601 cgtggcttgc cagccctgca aaggccatgc tatatacttg ctggatttca agtacttatc
      661 aatttgtggg atagcttcaa tacgtttttt aaaacaaact aattttggga acgcatccag
      721 gcacattggg tccatgtata aaacaacatc aagagcgtca tacaacatga agtcaggatg
      781 ggttacatga tcaccattta aatatgtt
//
