LOCUS       sequence-107311-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of eGFP in T. cruzi. The NheI-linearized form of this 
            plasmid could be efficiently integrated in to T. cruzi genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Peng D, Kurup SP, Yao PY, Minning TA, Tarleton RL
  TITLE     CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in 
            Trypanosoma cruzi.
  JOURNAL   MBio. 2014 Dec 30;6(1). pii: e02097-14. doi: 10.1128/mBio.02097-14.
  PUBMED    25550322
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2014-12-30"; volume: "6(1). pii: e02097-14. doi"; pages: " 
            10.1128/mBio.02097-14"
COMMENT     Sequence Label: pTrex-n-eGFP
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccttgctcac cattctagac aagacaactt atagagcaaa aaaaaaaaag aaaagacatc
       61 ataaaagaag ttggaaaaga aaaaaggtaa aaacaattta acacaacaat aataagtgga
      121 taaaacaaca aagaaaaagt attataataa caatcataat aatttcgctt tcgtgcgtga
      181 aaatgtttct tagtgaatcc gctgcatatt ttgaagaaac tcgttggggg atccactaga
      241 actcttgcac agacttggca tccacacggc gctttgactc cccttctgaa aaaaaaagaa
      301 cacgttgatt ccccttttga tttcccacga atgcgaagca caattcacgc acagccaacg
      361 gcagggagag acgataaggt aaaatacaca caagtggtgg aatcacaaaa ggacacgcac
      421 agcacgcaaa taaacattgc acacggcatg acattttgcg acccacacac aatgtgtaca
      481 ctacaattat atgaaaatcc ctcccccatt ccgcgggtgc gccgcaaagg gccaaaaacc
      541 caaatgatcc actttattat aaacctcact gaccaatgag ccaaaaagaa ggtcgcgccg
      601 cacacgatac ggcgatgcga aacaaaagac cgcaaaaaaa aaaacgaaaa accaaacatc
      661 ctgagggaca ccgattcctt ccgacgccac ctgggccacc ggagagaata ccgctccatg
      721 atgtactaga cacaaatgga cggttaaa
//
