LOCUS       sequence-107316-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    576..600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(600..619)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(601..619)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tccatgtgct ggcgttcaaa tttcgcagca gcggtttctt taccagactc gagggtacct
       61 taatcactcc gggaagcaag aatttgcttc agcagccgat ggaaatactg ctgctgggag
      121 ttgaggtagc gcttgtactg tgacttgaag cataactgcc ggtacttgac cacctcgggg
      181 ttaaagtgtc cgtctcggaa aagcttctgg gcaatgtgca gagggtttcc aaagcggaag
      241 ttctccccac tgaacaaggc taagatgagt tcattctgct gctcagcact gtcttcagga
      301 aactggggca gaaactgctg gaggtgttca cgttgagaat cacttaacac ttcctgccat
      361 gttgagaggc tgacaacatc aaagaagatc tcaggatcct ccagaaggtc ctcgggcaga
      421 ctaactctgg tgcctcccag gaggcaatcc tccatgatgc gcgtgccatg gccatctcca
      481 cacggaccaa gttccagagg atctgtcagc atatggtcta aggaatccat attatgtata
      541 tctccttctt atacttaact aatatactaa gatggggaat tgttatccgc tcacaattcc
      601 cctatagtga gtcgtattaa tttcgattat gcggccgtgt acaatacgat tactttctgt
      661 tcgacttaag cattatgcgg ccgccactgt gctggatatc tgcagaattc caccacactg
      721 gactagtgga tccgagctcg gtacctcatt tggtttcctg agctttcttt gcgttctgtt
      781 tttcttttgc cttttctact agtggtatta actgctggtg ttctgctaa
//
