LOCUS       sequence-107317-       16293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  helper plasmid for bacterial conjugal DNA transfer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16293)
  AUTHORS   Elhai J, Wolk CP
  TITLE     Conjugal transfer of DNA to cyanobacteria.
  JOURNAL   Methods Enzymol. 1988;167:747-54.
  PUBMED    3148842
REFERENCE   2  (bases 1 to 16293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "1988"; volume: "167"; pages: "747-54"
COMMENT     Sequence Label: pRL528
FEATURES             Location/Qualifiers
     source          1..16293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     218..237
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     9398..9417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9638..9655
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     9896..9918
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctgcaggtcg actctagagt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc
       61 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga
      121 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
      181 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
      241 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
      301 aactgaacac gtcgcgtgac cgtgaaaaca ccttcgaacc gcagttgatt aagaagcacc
      361 agacgcgcat tacgcagatg gacagccaga ttttatccct ctacgccaaa gatatgacta
      421 cccgcgaaat tgtcgccacc ttcaaagaga tgtacgacgc agatgtgtca cccacgctga
      481 tatgtaaagt cactgatgcg gtcaaagagc aggttgcaga atggcaaaat cgacctctgg
      541 atgcactgta tcccattgtt taccttgact gtatcgtggt gaaagttcgt caaggtggca
      601 ccgtaattaa caaagcggtg ttccttgcac tcggtattaa tactggaggc cagaaagagt
      661 tgctgggcat gtggctggcc gagaacgaag gggcgaagtt ctggctgggg gggctgacag
      721 agcttaagaa tcgggggctt caggatatcc ttattgcctg catggacggt ctgaaaggct
      781 ttccggatgc gataaacagc gtgtatccgt agacgcacat ccagttgtgc atcattcata
      841 tggtgcgcaa cagcctgaaa tacgtatcgt ggaaggacta caaagccgtc actggcgggt
      901 taaaaacggt gtatcaggcg ccaacagaag aggcggcgct agtggcaccg gagcagttcc
      961 cgaatgtctg ggacgataaa tacccgcaaa tcagcaaaag ctggcgtgcg cactgggaaa
     1021 atatcaacac gttcttcgct attcgcccga tatccgcaag gccatctaca ccacgaacgc
     1081 tatcgaatcg ctgaacagcg tgatccgtgc ggccattaag aagcgtaaag tgttcccgac
     1141 tgacgactcg ctgcggaagg tcatttctct ggcgatcaag gatgcatcaa aaaaatggaa
     1201 tatgccaatc cagagctggc ggctggcgat gagtcgcttt attatcgagt tcggtgaccg
     1261 cctgagcgat cacctttaat atggcagcaa ttacacagaa ttatttacag ggtctatctt
     1321 ttatttatct tatctaaaag atgtaccaca gatttgccaa cagcaaatga cagattaaca
     1381 ggaacagcgt ttcctatttg cttatatttc gctgacattg ggccttcaaa tacccattca
     1441 tcggggaagg tctgtattct tgcatactca cgcacagtta atggtcttgt ttcttctggg
     1501 tggcaacgct ctgtttgttt ctgtgctggg gcgcatgtta atgttaggct tggttcatcc
     1561 catgacaaac gacgagccat accagttttg cccccaccta agtaaaaact cttgagcatg
     1621 tattcttttt gaatatcttc aggaagatct ctccagtagc caccgggagg aaccatactt
     1681 aggatctctg cttttctttt gggatatttt tgtccttcag attctggcac gtcgctatca
     1741 tacagctctc ccttttttaa tgcatcttta agggttaata ttttattgta agaagaaggc
     1801 cactcataat cgatgccatc agcaagatca tttcttacag ctacaatgat caaacgctcg
     1861 cgtttttgcg gcactttgta gaaaatagcc ttaagcactc ttggctcaaa taaagtgtag
     1921 cccaagtctg tgataatatt ttttattgtt tctaaagttc gtccagcatc atgatttagc
     1981 aaccctcgaa cattctctgc taaaagaact ttcggattga tttctttagc ggctcgggcg
     2041 aattcaaaga aaagggtgcc ccgtgtatct tcaaaaccaa gttttttgcc tgcataagag
     2101 aatgcctggc aaggaaagcc accagccagc acatcaacgg tattcctata aggggtgaag
     2161 tctacttggc tcacatcacc ttcaaccaca ttccattcag gcctattttt tcgtaacgtc
     2221 ttacaagcat gggagtcaat ttcatttagt aaaacagatt ttaaaccggc tttttctaaa
     2281 cctaaagcca tcccccctgc gccagcaaat aactctaata cagtataaac attattgctt
     2341 attttagtct catcaggcca ctggctttta aataaaaact gagcttgttc aaaattttta
     2401 agttgctctt ttttataaaa tcgatagttg ttttcgtcat ttctttgaga aactaatttt
     2461 ccagcagtat cccaacgcct taaagtttct tttgaaacgc ccaaaatgtc tgcaacttct
     2521 gaaagtgaaa actcttcctt taacatattt agtaaccatg ccaacattag tcatggttac
     2581 tactgtatgg gtttacatgt gctttgtcaa acattgatca tggttatcag ccgtaagcga
     2641 gcccaaaata cttaataaaa ctatctttat cagggatgga aaaaccttgc tgcccaagac
     2701 gttcgctcaa aatatactca attgcgtgag ggagagcact atggagttct tgcagagcat
     2761 ctggtctgcc agtgacaagc tcatagaaac tcgcaccatc aatgattcta atgttagggt
     2821 tcgaaggaca cagagtacca ctacctttat tggaaggagt aaaggggctg ttatatctga
     2881 taggtttacg agggattata ttaacaaagt acgcacagta atccttaaat cggctatgtt
     2941 tcggtgatac caactcatct aagcctttat atttatctgc taaatccccg ccagtaaccg
     3001 ttgagtattt atttttcacc tcagcgatga ttcttctttc ttcgttgaga agatccacag
     3061 gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag
     3121 gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg
     3181 catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac
     3241 gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag
     3301 ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact
     3361 gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttctagagga tccccccttt
     3421 caatttggct tactcggatc tttaatgctc ttgcctgctc aacaaatggt gttgcttttc
     3481 gatttctttc taaagctaaa gcaacaaatc ctgctcggac ttcttgatat ggagtcacaa
     3541 gatcatcatt gctttgaata tgatattgat atggcactag ggttttctcc aaacatacac
     3601 acatttgcga agtgtctttc ttccatgtgt ccccatttgt tggctactgt tacctttgcc
     3661 agtaggtaag acaagaattt tctccacatt aaaaccaatt tcttctgcaa tattagaaag
     3721 aattaaatca accgaaatat caatacctgc atagcgaaca ttatcattta ccataaataa
     3781 aggtgagcca ttttttaaaa ctctaaagca ttctataata acgcaagcca tttcatagaa
     3841 atatcctttt atcatacgag gaataccgtt attattaagt ttttttcttt caagctctcg
     3901 ctcaagaaag cgcaagatac tttgtaacaa ttcttgttta tcaagtatgc gtaatgcttc
     3961 ctgccaattg tgaataagag acttttcttt gttttcaaca gtacaactca gcatatcttg
     4021 cctaagttgt actatatctc tttctcccac acctaataga gctaattcta gagcgtatgt
     4081 acgtgtatag tcataacgat tacaataggg tggagaggta atgatacagt cgtaaaaatc
     4141 ttgctcaaat tcaggcagaa ttttaagaca tgaagcatta aataaattaa ttccacttct
     4201 ttgagtccca taacaaaata atgtattact tattaagtca aaggaatcat tcaaaattaa
     4261 ttttatttgc tcagtaattg cttcatcgaa atccagaatt ttacctttat caaatttatc
     4321 tgatccactt ttcctaaatg ctcttttatc ccaacgtaga tactgtccat ctttacgggt
     4381 atagctgata gattcaagaa tacacaatag agcaaaacgg agaacttgtt tcacaagaat
     4441 attctctttt tctatagaaa ataaaaatct ctctattgat gcttctgttt caggaggata
     4501 agctccgtca gtaattctta aacgattaag atattttcta ttattatgct gattccaagg
     4561 tttttgtttg taccattcaa tcaatctcaa aaaatcttct cgacggaatt gtcgctggat
     4621 tattttcctt acttcaataa tctcttgacc aataggtaac acttctatac catctgcttg
     4681 aataccggca tcactacagg caaatagcat tgttcctgct cctgcgaagg ggtcaagaat
     4741 cctctcaaat gattttttct cgtactcgaa tattaactga tttaccaaat caactgaaaa
     4801 agcttcttta tatttaaacc agcgatactg aggttttgac ttattagctt gaaaactaac
     4861 tatttttcga cttaaacttt catgcaatcg catcttatct ctaaaacgtt gctcaagtgc
     4921 tttattgaat cgagaaattt cttgtacaaa ggaatcactc tcttgatcaa gatcagtcgg
     4981 gtcagtcttt atttctattg gactctcaag ctcaaatgaa gtcattttat atttttcttg
     5041 taacaaagag gtaacttaag attctatcag gacaaagcac tatcgtctat cacttaagat
     5101 ttgaacccta ttttttatag actaattgac aacagttaga acctgattat actttgttat
     5161 acttctacag taatcaagtt aaatattgaa tagggtaccg agctcgaatt catcgatatc
     5221 tagatctcga gctatcaggc tagagatttt accgccaaat cgaaccttat tagagcggtt
     5281 taggctggac cggcagttaa aattggggct tgagcggtaa acgagtgagg gaatttcagg
     5341 taagatactt cggatgagga gcaaaaaggt ggtttatact tcctataccc aaaaaggtgg
     5401 tttatacttc ctataccccg gttatccggt ggttttctgt ttgcggaata aaacagatta
     5461 gtgacagttt tctcagaatg gcgtgttccg gtgacttctg ttttcgtgtg ctgcatccgg
     5521 aagtgtcagg cccgccggat atgaaaaagg cctccggaga gtaccgtgac tttattttcg
     5581 gcacaaatac aggggtcgat ggataaatac ggcgatagtt tcctgacgga tgatccgtat
     5641 gtaccggcgg aagacaagct gcaaacctgt cagatggaga ttgatttaat ggcggatgtg
     5701 ctgagagcac cgccccgtga atccgcagaa ctgatccgct atgtgtttgc ggatgattgg
     5761 ccggaataaa taaagccggg cttaatacag attaagcccg tatagggtat tattactgaa
     5821 taccaaacag cttacggagg acggaatgtt acccattgag acaaccagac tgccttctga
     5881 ttattaatat ttttcactat taatcagaag gaataaccat gaattttacc cggattgacc
     5941 tgaatacctg gaatcgcagg gaacactttg ccctttatcg tcagcagatt aaatgcggat
     6001 tcagcctgac caccaaactc gatattaccg ctttgcgtac cgcactggcg gagacaggtt
     6061 ataagtttta tccgctgatg atttacctga tctcccgggc tgttaatcag tttccggagt
     6121 tccggatggc actgaaagac aatgaactta tttactggga ccagtcagac ccggtcttta
     6181 ctgtctttca taaagaaacc gaaacattct ctgcactgtc ctgccgttat tttccggatc
     6241 tcagtgagtt tatggcaggt tataatgcgg taacggcaga atatcagcat gataccagat
     6301 tgtttccgca gggaaattta ccggagaatc acctgaatat atcatcatta ccgtgggtga
     6361 gttttgacgg atttaacctg aacatcaccg gaaatgatga ttattttgcc ccggttttta
     6421 cgatggcaaa gtttcagcag gaaggtgacc gcgtattatt acctgtttct gtacaggttc
     6481 atcatgcagt ctgtgatggc tttcatgcag cacggtttat taatacactt cagctgatgt
     6541 gtgataacat actgaaataa attaattaat tctgtattta agccaccgta tccggcagga
     6601 atggtggctt ttttttatat tttaaccgta atctgtaatt tcgtttcaga ctggttcagg
     6661 atcactgtac gataatgccc ccgcagtttg gtaataccct taataaaaaa gaaacagcaa
     6721 agactgacag caataataat aaagtaagca gtaacaataa tattaacaac accagatgca
     6781 gttataataa tagtatttaa gacaccagaa agactgctgc gacagtcatt ttgaacaaca
     6841 ccaaaatgcc gtaaaggcag tagtaacaac accagtgaaa acatcacgat agcatagtga
     6901 tatgcctgag tgtgtgtaat taaacaataa ataaaccgcc atatataaca gaagatagta
     6961 ttctgaatgg catgcttttc tgttcagtat aaacatatca tcccggttgg tataaggatg
     7021 atatataata agttaagctg aacacatatt tattttggtt ttattttaca aataaagtaa
     7081 gacgatccgt taagtcaaag cggggtatat ttattatacc ctgctttttt atttgtccgc
     7141 cgggcgcgga taatggatca gattatgcag tgtcacaatg gccttaccgg gattggcgta
     7201 agcgtgcggg atatccgcat ggaagcgcag ggattccccg gcagaaacgg tgtgccactc
     7261 atcccccagc cgcagttgta atgcgccttc cagtacaatg acatgttctc tggttctgaa
     7321 atccatccct gtcggtgttg cttatgcagt ctggtcggga ctcggcgtcg tcataattac
     7381 agccattgcc tggttgcttc atgggcaaaa gcttgatgcg tggggctttg taggtatggg
     7441 gctcataatt gctgcctttt tgctcgcccg atccccatcg tggaagtcgc tgcggaggcc
     7501 gacgccatgg tgacggtgtt cggcattctg aatctcaccg aggactcctt cttcgatgag
     7561 agccggcggc tagaccccgc cggcgctgtc accgcggcga tcgaaatgct gcgagtcgga
     7621 tcagacgtcg tggatgtcgg accggccgcc agccatccgg acgcgaggcc tgtatcgccg
     7681 gccgatgaga tcagacgtat tgcgccgctc ttagacgccc tgtccgatca gatgcaccgt
     7741 gtttcaatcg acagcttcca accggaaacc cagcgctatg cgctcaagcg cggcgtgggc
     7801 tacctgaacg atatccaagg atttcctgac cctgcgctct atcccgatat tgctgaggcg
     7861 gactgcagat ggaatgcgct ttgttacgcc agtcagaaca atcaatgccg gaccgaaccg
     7921 caaatacttt ggtaataaca gagggatcac ctggtacaac tttgtgtccg atcagtattc
     7981 cggctttcat ggcatcgtta taccggggac gctgagggac tctatctttg tgctggaagg
     8041 ccttctggaa caggagaccg ggctgaatcc aaccgaaatt atgaccgata cagcaggtac
     8101 cagcgaactt gtctttggcc ttttctggct gctgggatac cagttttctc cacgcctggc
     8161 tgatgccggt gcttcggttt tctggcgaat gggccatgat gccaactatg gcgtgctgaa
     8221 tgatattgcc agagggcaat cagatccccg aaaaatagtc cttcagtggg acgaaatgat
     8281 ccggaccgct ggctccctga aactgggcaa agtacaggct tcagtgctgg tccgttcatt
     8341 gctgaaaagt gaacgtcctt ccggactgac tcaggcaatc attgaagtgg ggcgcatcaa
     8401 caaaacgctg tatctgctta attatattga tgatgaagat taccgccggc gcattctgac
     8461 ccagcttaat cggggagaaa gccgccatgc cgttgccaga gccatctgtc acggtcaaaa
     8521 aggtgagata agaaaacgat ataccgacgg tcaggaagat caactgggcg cactggggct
     8581 ggtcactaac gccgtcgtgt tatggaacac tatgtatatg caggcagccc tggatcatct
     8641 ccgggcgcaa ggtgaaacac tgaatgatga agatatcgca cgcctctccc cgctttgcca
     8701 cggacatatc aatatgctcg gccattattc cttcacgctg gcagaactgg tgaccaaagg
     8761 acatctgaga ccattaaaag aggcgtcaga ggtagaaaac gttgcttaac gtgagttttc
     8821 gttccactga gcgtcagacc cctgtacaag gaggaaccgt tgcaccatct tcgtcttctg
     8881 aactgacggg gatcgcggtt cagtagaaaa gatcaaagga tcttcttgag atcctttttt
     8941 tctgcgcgta atctgctgcc tgtaaacgaa aaaaccgcct ggggaggcgg tttagtcgaa
     9001 ggttaagtca gttggggact gcttaaccgg gtaactggct ttagtggagc acagatacca
     9061 aatgctgtcc ttccagtgta gccgctataa ggcttcaact tcaagagttc tgcgtatcta
     9121 aatccactat ccagttacca atggctgctg ccagtggcgt taagtcgtgt cttaccgggt
     9181 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9241 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccaa cagcgtgagc
     9301 tatgagaaag cgccacgctt cccaaaggga gaaaggcgga caggtatccg gtaagcggca
     9361 gggtcggaac aggagagcgc acgagggagc caccaggggg aaacgcctgg tatctttaag
     9421 tcctgtcggg tttcgccacc tctgacttga gcgtcggttt ttgtgatgct cgtcaggggg
     9481 gcggagccta tggaaaaacg ccagctgcgt ggccttttcc gggttcttgt tttttgctca
     9541 catgtttctt ttggtgttat cccctgattc tgtggataac cgcattaccg tctttgagtg
     9601 agctgatacc gctcgccgca gccgaacgac cgagcggagc gagtcagtga gcgaggaagc
     9661 ggaaaagcgc ctggacgagc attttctcct tacgcatctg tgcggcattt cacacccggc
     9721 atggtgcact tttcatacaa tcctcactga tgccaaatga ttaagccagt atacactccg
     9781 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgctaaaacc cgctgacgcg
     9841 ccctgacggg cttgtctgct cccggcatcc gctcacaggc cagctgtgac cgtctccggg
     9901 agctgcatgt gtcagaggtt ttcaccgtca tccccgaagc gtgcgaggca gctgcggtaa
     9961 agctcgtcag cgtggtcgtg aagcgcttcc agatgtcagc ctggtcgtcg gcgtccagtt
    10021 cattcagttt ttccagcagg gtcagcgtct ggcttctgat gaagcgggcc atgttaaggg
    10081 cggttttttc ctgtttagtc acagttctgt ttctccatgt gtggggagtc tgtcggaggt
    10141 ttttaatgat accggtgaaa cgcgaaagga tgctcacgat acgggtcacg gatgaggaac
    10201 atgcgcggct gctggcacgt tgtgagggaa aacaacttgc gtcatggatg cgtaaggtct
    10261 gcctggggca ccgccatcga aaacgtcagg tcttccgaca ctggcaccgc cgctgttgcg
    10321 ccagttcgct tcggtcggca ataacctgaa tcagatagcc cggaagataa acagcggaca
    10381 gtggtcgggg catgaccggg ttcatgtggt ggctgcactg atggccatcg gacgtgaact
    10441 gagtgagctg agggatgagg tccgtaaaca gggagaacgg gatgatagtt aagtttcatg
    10501 ccaggggaaa aggcggtggc agtggtccgg ttgattacct gctgggccgg gagcgtaacc
    10561 gggaaggggc gagagttctg cggggtgctc ccgaagaggt gcgggaactc atcgatgcca
    10621 cgccgtttgc gaaaaaatac acctcggggg ttctgtcttt tgcagagcag accctgccac
    10681 cgggagagcg tgaaagggtg atggagagct ttgagcgggt actgatgccc ggtcttgaaa
    10741 aaaatcagta cagcatcctg tgggtggagc accaggacaa ggggcgtctt gagctgaatt
    10801 ttgtcatccc gaatatggag ctggcgagcg gaaaacggct ccagccctat tacgaccgag
    10861 cggaccgtcc gcgaatcaat gcctggcaga cactggtgaa tcaccattac gggctgcacg
    10921 acccgaacgc accggagaac aggcggacac ttgtcacccc gaataatctg ccgaaagcga
    10981 aacaggaggc cgcagaagcg attacgaggg gactggaggc tctttaccat gccggagcgc
    11041 taaaaacgcg tcaggatgtc actgaggcgc ttacagcggc aggttttgag gttgtgagga
    11101 caaccaggag cagtatcagt atcgccgacc cggagggtgg gcgtaatctc aggctgaaag
    11161 gggcgctgta tgagcaatct tttacaaatg gggacagact tcgagaagaa actgaaagag
    11221 cgtgccgtct ctacagagaa catgctgaac agcgagttca ggaagctcga agcgtctgta
    11281 gacagggaac tgaacttaaa cgggcagaaa atcagagacg ctatcagcgc acacacagca
    11341 gcggtgaaag aacaactgga gacgctgaac agtgccgtga acgcacagtt cagcgccaca
    11401 gagaacgaac tggcccggcg gcaggaagag ctgctctgga agctggtcaa agggcggata
    11461 ctgtacccgt cgctgacggc gctttgcgtc acgggcggga tttttctggc gtcatggggg
    11521 ctgattcagt ggcaggaaag ccggattgcg ggaaacatcc tggctatcag ggaccaggaa
    11581 gagacgctgg cgaaactccg ggaaaaaaca tggggtgtga cgtatcacga gggcaggaac
    11641 gggaaatttc tggtgctgcc gtccggggtg aagggggaaa acaactggac ggtggagaag
    11701 aagaacgcag taagactggt cagggagtaa gtgagtatga cagagctgga aatgcactta
    11761 ctgaacgcct tagagcaatt gcagcaggac tatacgcagc ggctgaaaga ctgggagagc
    11821 cccttcacgg atttgcagca tatgttcgag ctgacacgac aggagaacgg gcaactgagg
    11881 gaacagtgcg acgacttgag tcagcaggtg cagagattag ccgggcaggt ggaccacttg
    11941 agtcggttat ttatcacgaa cagcagataa gggatgaaca ggaaaggcag gcacgcctgg
    12001 aacgggagcg tgcgcggtcc tggcatgaag agcaggaacg ggaagcacgg gtatggcgtg
    12061 gcccgtcaat agggatgtga cggtgagttg ttccgggagg ttgccagatg gtaccggaac
    12121 gcgttaaaaa cgcatccggg cgcgattctg gcgtgtcgtt tttgttgctg tgttttgcct
    12181 ctttgccaga tcgcgctcat tcctcagcct catcccgcga tgtctgcgta tgtcatcaac
    12241 tgaaaagatg gaaaaaaaac agtgtcatga ggtttacgca ggcaaaacca gcgttattca
    12301 catggctgaa tagctatgca tacggttaaa atttatcagg tgcgatcgcg gcagcttttc
    12361 gggtggtttg ttgccatttt tacctgtcag cagccgtgat cgcgctgaac gcatttcagc
    12421 ggtgcgtaca attaagggat tatggtaaat cccttactgt ctgccctcgt atccatcgag
    12481 ataaaccgca gaaatggtgt ctgccattag tttcattttt ttcggcgaag catatcgctg
    12541 tttgtgtatt ttctgccagc aaaggctgtt gtgctccgga gtgagtgaaa aaatttttct
    12601 gagtggaatt cttttacacg ttgctgtgcc gtttctgctg atactccggc tgctttgtct
    12661 ttcatcccca tatcccggaa ctgctgaaag aacgggagca tcatacgtat ggtgtctgct
    12721 ttctgggtgt agaagttagg atcaaatcca tcatctgaag cataaacata atgccgggta
    12781 tgtcgttcca ggctggtctt tggtgtgcat cctgtaagag ctattgttgc tgttatcaga
    12841 ggtatcagaa gcagcctggc agcggcagat ctcataaaca tctccttgcg tttttaatct
    12901 tgatactcag ggagttatat ctccgaagag tatctcttat catacgtcgg tttactccca
    12961 atgtgatttg atggtcaatg agtcaggaat aatcccgtga aaaaatgtgt gccagtcctg
    13021 ctttttagtg ttctttctgc ttcggttgcc agtcatgcgg acagcaatgt ttttcaacaa
    13081 aaggccgaga agacaaaaaa cggcattgtt gcggatgtta atgccagaca gaaaatgttc
    13141 agtaacagac tgaagataca acttcagatc gatgagttga gagcagactg gctggcacca
    13201 gagatgccca aaaaagagag gatttgcgta aaaaactgga gtctctggag cggcaaaaga
    13261 aatccttacg ctgatggggg cgtattttcc ccggtttctt ccataacctt tttaccgttc
    13321 agatatttac gggcgaggcg aatgcgtctt gcctggtata ccgccgagta agcctgggtg
    13381 ggattcacac cattactgat ttcactgacc acatgacgct gatcagccag tgtcagcgtg
    13441 taaacctgtt tctggatgag gtcacgggct ttgtcatgct catatccggc agcaacaagg
    13501 gtgtcgagaa gtcgtttgtc tgttttgttc agtttatggc ggggtgattt gctcacatcg
    13561 ttctccgttt tttcatgatc caacagtttt atatctcatc ctgatttgct ccggtgttct
    13621 gtttcgacgt tctcaatgcc tgcctcccca tagtgtgatc cttattgcaa aaatgaaaat
    13681 ccatgctctt gacatggaca atgctgagta gtaggttttt actgtacata aaaccagtgg
    13741 ttatatgtac agtatattgt ttttaattta ttgttttaat tatcaaagag gaatttatgg
    13801 ctaaagaact aagtggatat ggaccaactg ctggtgagtc tatgggggga acaggggcga
    13861 atctgaatca gcaaggtgga aataataata gtaatagtgg ggttcactgg ggtggtggct
    13921 ctgggcatgg taataatggt ggtcagggaa attcaaattc atctggttct acatcaactg
    13981 tgatgaaaac cggtgagtca tatctgactc cgtggggtga tgttgtcatt aataatgatg
    14041 gccttccggt gatgaatggt attgttatga cagaagagaa ttcaactctg gttgataatc
    14101 catttggagg ggtctccaga gttcttaatt ctttaattag tgatatgcca tcactttttg
    14161 cagaaagctc aggaaataac aacaataaca ctgcttcagt taatactgca ccaacgaatg
    14221 ctcaggtcag tgatatggat aagagttcga aggtggtggg taatgtaatt aatgaaaaac
    14281 aaaagcagaa gaataaaatt gctacacaga ttagtgaaaa acagaagaaa atcgaggaga
    14341 tgaaaaaagt attcaagcat cattcttatc atggtatcac tgatcttgag agggatgttg
    14401 atgagttgca aaaaaaatca aatcagcttg atgcagatat tagcaaactt aattcttata
    14461 aaaatacatt gcaaagtaaa attggtgatg tgaataaaca gaaggaagct gaagaaaagg
    14521 ctcgtgaaaa tgcagaggtc gctgaacatg aaactcttaa tgaagagaaa caggcggtgg
    14581 cggaagcaga gaagcgttta gctgaggcga aagctgaact ggcgaaggct gaaagtgatg
    14641 tacagagtaa gcaagctact gtttccagag ttgcaggaga gcttgaaaac gctcagaaga
    14701 gtgttgacgt gaaggtcacc ggatttccgg gatggcgtga cgttcagaaa aaactacagc
    14761 gtcagcttga agctaagcag gctgagtact ccgcagttga aaatgaactt aagaatgctg
    14821 ttagtttcag ggatggtaag gccgctgagg ttaaagaggc agagcagaaa ttaaaagaag
    14881 ctcaggatgc tcttgaaaag agccagataa aagatgctgt tgatactatg gttggatttt
    14941 atcaatatat aaccgaacaa tatggagaaa aatacgcaaa aattgctcag gatttagcgg
    15001 aaaagtctaa aggaaagaaa attcaaggtg ttgatgaagc tctggcagca tttgaaaagt
    15061 ataagaatgt actggataag aaattcagta aggttgatcg ggatgctatt tttaatgcgt
    15121 tagagtctgt taattatgat gagttatcta agaatttaac gaagatatca aagtcgctta
    15181 aaatcaccag tcgagtatct tttctatatg atgttggttc tgattttaaa aatgcaatag
    15241 aaactggtaa ctggcgacct ttgtttgtca cgctggagaa aagtgcggtc gatgtaggtg
    15301 tggcaaaaat tgtggcatta atgtttagtt ttattgttgg tgtgcctctt ggtttctggg
    15361 ggattgcaat tgtcacaggt attgtttctt cttacatagg ggatgatgag ttgagcaaac
    15421 ttaatgagtt gttagggatt taatttctct ttagagtgta atatatgaaa cttggcaatc
    15481 ctactgggat tgccattaat aaataaaaca atgcagataa cttgcataag taccagggtt
    15541 ctttttttaa ctttgtattt tctttgatat atctagatat ggttaattta gagtatggat
    15601 atagcaatac atgtattgat gatataaata taaataacaa aggagtgtta tcattgtcgt
    15661 taaatattaa aataagaatc catggtataa gtgattcagg taaattatgt aggtagtatt
    15721 ttaaatgcat aagtattttt acttttgttg gaaagaaata tgaaaataat aatttttcta
    15781 attacaatat tattcttgtc aggatgtcaa gcaaactata ttcgtgatgt acggggtctg
    15841 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    15901 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    15961 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    16021 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    16081 agggcttacc atctggcccc agtgctgcaa tgataccgcg agatccacgc tcaccggctc
    16141 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    16201 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    16261 cagttaatag tttgcgcaac gttgttgcca ttg
//
