LOCUS       sequence-107318-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hUb in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pET3a-hUb
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..244)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     RBS             252..274
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(305..324)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(306..324)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(408..427)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agcagccgga tcctcaacca cctcttagtc ttaagacaag atgtaaggtc gactccttct
       61 gaatgttgta atcagacagc gttctaccgt cctcgagctg cttaccggca aagatcaatc
      121 tttgttgatc aggtggaatg ccttccttgt cttgaatttt agccttaacg ttttcgatgg
      181 tatcggatgg ttcaacttct agagttatgg ttttaccggt taacgtcttg acgaagatct
      241 gcatatgtat atctccttct taaagttaaa caaaattatt tctagaggga aaccgttgtg
      301 gtctccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg
      361 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
      421 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
      481 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
      541 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
      601 ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
      661 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
      721 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
      781 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
      841 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
      901 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg t
//
