LOCUS       sequence-107319-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGS
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..49)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    584..608
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(608..627)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(609..627)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta ccagactcga
       61 gggtacctta atcactccgg gaagcaagaa tttgcttcag cagccgatgg aaatactgct
      121 gctgggagtt gaggtagcgc ttgtactgtg acttgaagca taactgccgg tacttgacca
      181 cctcggggtt aaagtgtccg tctcggaaaa gcttctgggc aatgtgcaga gggtttccag
      241 agccggagcc ctccccactg aacaaggcta agatgagttc attctgctgc tcagcactgt
      301 cttcaggaaa ctggggcaga aactgctgga ggtgttcacg ttgagaatca cttaacactt
      361 cctgccatgt tgagaggctg acaacatcaa agaagatctc aggatcctcc agaaggtcct
      421 cgggcagact aactctggtg cctcccagga ggcaatcctc catgatgcgc gtgccatggc
      481 catctccaca cggaccaagt tccagaggat ctgtcagcat atggtctaag gaatccatat
      541 tatgtatatc tccttcttat acttaactaa tatactaaga tggggaattg ttatccgctc
      601 acaattcccc tatagtgagt cgtattaatt tcgattatgc ggccgtgtac aatacgatta
      661 ctttctgttc gacttaagca ttatgcggcc gccactgtgc tggatatctg cagaattcca
      721 ccacactgga ctagtggatc cgagctcggt acctcatttg gtttcctgag ctttctttgc
      781 gttctgtttt tcttttgcct tttctactag tggtattaac tgctggtgtt ctgctaa
//
