LOCUS       sequence-107332-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB9 (small HSP) tagged with V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-V5-hspB9
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(517..558)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(661..685)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    695..713
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    716..734
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(736..835)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(765..785)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tcgattttgg cgcgtttacc gggtcaggtt ggaagcctta gagccgaggc tcccgagtct
       61 cggggacggt cctgtttggg cttcagggag ggccagcgcc acacactggc ctctgaccca
      121 cagctgcccg gagggggtca ggcagcaggt catggcggta ggactcaggt tggacgggag
      181 cattttccgg tgcaccttct gtgacatgcg gtaactgacc ctttccgggt ccctgacgtc
      241 cagttgctgc tgtccggtca ccatcagcca ttggccatcc acctgcacca ccagttcctc
      301 cggggcgaag ccgtgggcat ccagcttcat ttggaaaccg tctctggcat ggtcattgtc
      361 ctcctgagcc gctggactgt ccctgagcag ccgcaccggc attgtggcca cccggttccg
      421 ttcagcaagg cccacgctgg gacaccggga tgccacccgg ctctcgttgg agaaggtgtt
      481 accgactctc tgcatggttc tcgaggtttt ggatcccgta gaatcgagac cgaggagagg
      541 gttagggata ggcttaccca tggtgaattc aagcttaagt ttaaacgcta gagtccggag
      601 gctggatcgg tcccggtgtc ttctatggag gtcaaaacag cgtggatggc gtctccaggc
      661 gatctgacgg ttcactaaac gagctcgtcg acgatctcta tcactgatag ggagatctct
      721 atcactgata gggagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
      781 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
      841 aaacaaactc ccattgacgt caatgg
//
