LOCUS       sequence-107334-        5952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Photoactivatable transcription system. Luciferase reporter under the
            control of the Neurog2 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.12.011"; journalName: "Chem Biol"; date: 
            "2015-02-19- 19"; volume: "22"; issue: "2"; pages: "169-74"
COMMENT     Sequence Label: pNeurog2-Luciferase reporter
FEATURES             Location/Qualifiers
     source          1..5952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      218..227
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1907..2028
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1918..1937)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1972..1991
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2222..2579
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2222..2413
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2245..2265)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2430..2565
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2492..2511
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2610..3647
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             3626..3634
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    3671..3719
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(3875..3892)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4046..4634)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4126..4145)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4834..5625,5626..5694))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5799..5847
                     /note="synthetic polyadenylation signal"
     misc_feature    5861..5952
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5901..5920
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtaccg agtttctaga cggagtactg tcctccgagc ggagtactgt
       61 cctccgactc gagtgaatga tgataatacg atgcgggaat tccggagtac tgtcctccga
      121 agacgctagc ggggggctat aaaagggggt gggggcgttc gtcctcactc tagatctgcg
      181 atctaagtaa gcttggcaat ccggtactgt tggtaaagcc accatggaag atgccaaaaa
      241 cattaagaag ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct
      301 gcacaaagcc atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca
      361 tatcgaggtg gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc
      421 tatgaagcgc tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt
      481 gcagttcttc atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa
      541 cgacatctac aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt
      601 attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat
      661 acaaaagatc atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac
      721 cttcgtgact tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt
      781 cgaccgggac aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa
      841 gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat
      901 cttcggcaac cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg
      961 cttcggcatg ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta
     1021 ccgcttcgag gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct
     1081 gctggtgccc acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct
     1141 aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc
     1201 cgtggccaaa cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac
     1261 cagcgccatt ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt
     1321 gcccttcttc gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca
     1381 gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga
     1441 ggctacaaac gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg
     1501 ggacgaggac gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg
     1561 ctaccaggta gccccagccg aactggagag catcctgctg caacacccca acatcttcga
     1621 cgccggggtc gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt
     1681 gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt
     1741 tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact
     1801 gaccggcaag ttggacgccc gcaagatccg cgagattctc attaaggcca agaagtaagg
     1861 ccgcgactct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat
     1921 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     1981 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2041 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     2101 aacctctaca aatgtggtaa aatcgataag gatccgtttg cgtattgggc gctcttccgc
     2161 tgatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttagctacct
     2221 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     2281 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     2341 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2401 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     2461 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     2521 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     2581 agctcgattc ttctgacact agcgccacca tgaagaagcc cgaactcacc gctaccagcg
     2641 ttgaaaaatt tctcatcgag aagttcgaca gtgtgagcga cctgatgcag ttgtcggagg
     2701 gcgaagagag ccgagccttc agcttcgatg tcggcggacg cggctatgta ctgcgggtga
     2761 atagctgcgc tgatggcttc tacaaagacc gctacgtgta ccgccacttc gccagcgctg
     2821 cactacccat ccccgaagtg ttggacatcg gcgagttcag cgagagcctg acatactgca
     2881 tcagtagacg cgcccaaggc gttactctcc aagacctccc cgaaacagag ctgcctgctg
     2941 tgttacagcc tgtcgccgaa gctatggatg ctattgccgc cgccgacctc agtcaaacca
     3001 gcggcttcgg cccattcggg ccccaaggca tcggccagta cacaacctgg cgggatttca
     3061 tttgcgccat tgctgatccc catgtctacc actggcagac cgtgatggac gacaccgtgt
     3121 ccgccagcgt agctcaagcc ctggacgaac tgatgctgtg ggccgaagac tgtcccgagg
     3181 tgcgccacct cgtccatgcc gacttcggca gcaacaacgt cctgaccgac aacggccgca
     3241 tcaccgccgt aatcgactgg tccgaagcta tgttcgggga cagtcagtac gaggtggcca
     3301 acatcttctt ctggcggccc tggctggctt gcatggagca gcagactcgc tacttcgagc
     3361 gccggcatcc cgagctggcc ggcagccctc gtctgcgagc ctacatgctg cgcatcggcc
     3421 tggatcagct ctaccagagc ctcgtggacg gcaacttcga cgatgctgcc tgggctcaag
     3481 gccgctgcga tgccatcgtc cgcagcgggg ccggcaccgt cggtcgcaca caaatcgctc
     3541 gccggagcgc agccgtatgg accgacggct gcgtcgaggt gctggccgac agcggcaacc
     3601 gccggcccag tacacgaccg cgcgctaagg aggtaggtcg agtttaaact ctagaaccgg
     3661 tcatggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtgt
     3721 tcgaactaga tgctgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     3781 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     3841 cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3901 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3961 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4021 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4081 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4141 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4201 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4261 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4321 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4381 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4441 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     4501 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4561 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4621 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4681 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4741 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4801 acagcggccg caaatgctaa accactgcag tggttaccag tgcttgatca gtgaggcacc
     4861 gatctcagcg atctgcctat ttcgttcgtc catagtggcc tgactccccg tcgtgtagat
     4921 cactacgatt cgtgagggct taccatcagg ccccagcgca gcaatgatgc cgcgagagcc
     4981 gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca gcagggaggg ccgagcgaag
     5041 aagtggtcct gctactttgt ccgcctccat ccagtctatg agctgctgtc gtgatgctag
     5101 agtaagaagt tcgccagtga gtagtttccg aagagttgtg gccattgcta ctggcatcgt
     5161 ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct ggttcccagc ggtcaagccg
     5221 ggtcacatga tcacccatat tatgaagaaa tgcagtcagc tccttagggc ctccgatcgt
     5281 tgtcagaagt aagttggccg cggtgttgtc gctcatggta atggcagcac tacacaattc
     5341 tcttaccgtc atgccatccg taagatgctt ttccgtgacc ggcgagtact caaccaagtc
     5401 gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta tacgggacaa
     5461 caccgcgcca catagcagta ctttgaaagt gctcatcatc gggaatcgtt cttcggggcg
     5521 gaaagactca aggatcttgc cgctattgag atccagttcg atatagccca ctcttgcacc
     5581 cagttgatct tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa
     5641 gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt
     5701 cctttttcaa tattattgaa gcatttatca gggttactag tacgtctctc aaggataagt
     5761 aagtaatatt aaggtacggg aggtattgga caggccgcaa taaaatatct ttattttcat
     5821 tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa
     5881 acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca
     5941 gaacatttct ct
//
