LOCUS       sequence-107340-        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of MBP-MBK-2 K196R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW4.17
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..49
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gaacaacaac aacaataaca ataacaacaa cctcgggatc gagggaagga tcacaagttt
       61 gtacaaaaaa gcaggcttaa tgacactttt tgagccgtca acatcgggca atcgaatggg
      121 gtatagaggc tcgtccaact cgtcatctgg tgttggatcg ggaggatctg gaagtctgat
      181 gacgcaatcg atcggtggac ccaataaaca tttgtcagcg tcacactcaa cactaaatac
      241 tgcatcaact cacgatatga tgcattcgaa gattccaaag agcccgagca atgagagttt
      301 aagccgatca cacacatcct cgtcgggagg cagtcaaggt ggacacaatt cgaattctgg
      361 ttcaaactct ggtttccgac cggaagatgc tgtacagacg tttggcgcga agctggtgcc
      421 gtttgagaaa aatgagattt acaactatac acgggtcttt ttcgtcggat cacacgctaa
      481 aaagcaggcc ggtgtgattg gtggtgcgaa taatggtgga tatgatgatg aaaatgggtc
      541 ctatcagctt gttgtacacg atcatattgc gtaccggtac gaggtgctca aggttatcgg
      601 aaaaggatcg tttggacagg taatcaaggc attcgatcac aaatatcaac aatatgtggc
      661 tctgaggctt gtaagaaatg agaagcgatt ccatcgtcag gctgacgagg agattcgaat
      721 tcttgatcat ttgcggcggc aggattccga tggaacacac aatattatcc atatgctcga
      781 ctatttcaat ttccgtaatc acaagtgcat cacgtttgag cttctcagca tcaaccttta
      841 cgagttgatc aagaggaaca agttccaagg attctcactg atgctcgtga gaaaattcgc
      901 ttattctatg ctcttgtgtc tggatctttt acaaaagaac aggctgattc attgtgattt
      961 gaagccggaa aatgttctgt tgaagcaaca aggacggagt ggaataaaag tcatcgattt
     1021 tggttcatct tgcttcgacg atcaacgtat atacacatat att
//
