LOCUS       sequence-107345-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB4 (small HSP) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-hspB4
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(680..704)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    714..732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    735..753
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(755..806)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(784..804)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gactcgattt tggcgcgtcc gaggcaatgc ctgcttagga cgagggagcc gaggtgggct
       61 tctcctcccg cgacacgggg atggctcgct cggcgtgggt ggcatccagg ccagtctgga
      121 tcttggggcc acagaaggtc agcatgccat cggcagacag ggagcaagag agggccgact
      181 ggtccacgtt ggacggcagg cggtagcggc ggtggaactc acgggaaatg tagccgtggt
      241 cgtcctggcg ctcgttgtgc tttccgtgga tctccacaaa gtcgtcctgc accttcacgg
      301 tgaggtcctc cggggagaag tgcttcacat cgaggaagat gacgaacttg tcccggtcgg
      361 atcgaacctc agagatgccg gagtccagca cggtgcggaa gagggactgg cggtagtagg
      421 ggctgatggt ggacgacagg aagggcagca ggtcatactc aaaaaggccc tcgccgaaaa
      481 actggtcgaa cagccggctg gggtagaagg gccccagggt gcgcttgaac caggggtgct
      541 ggatggtcac gtccatggtt ctcgaggttt tggatccgag ctcggtacca agcttaagtt
      601 taaacgctag agtccggagg ctggatcggt cccggtgtct tctatggagg tcaaaacagc
      661 gtggatggcg tctccaggcg atctgacggt tcactaaacg agctcgtcga cgatctctat
      721 cactgatagg gagatctcta tcactgatag ggagagctct gcttatatag acctcccacc
      781 gtacacgcct accgcccatt tgcgtcaatg g
//
