LOCUS       sequence-107346-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HSPB3 (small HSP) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-hspB3
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(617..641)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    651..669
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    672..690
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(692..791)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(721..741)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 actcgatttt ggcgcgttct gaacaggaac cgatacgatg tcacttagtc ccaactggat
       61 cctttacttc caccaccaaa attccatcat gacagaggac tgcagacaaa tctttgattt
      121 ccacgccatc tggtagtttg tactgtcggg tgaagcttct tgagataaaa ccgtgctcat
      181 ccattctggt tccgtgttgt gcttttatca gtagccagcc ttcgaaggtc tgaatgatga
      241 tgtcttcagg gaggaactgg accacgtcca gcaggatctg aaagtgggat ttgccttctc
      301 ggggtggcgt ctctgccgct gagtccactg gaggagactg cgctgccctg gttttcctca
      361 ggtccacgat ggttggccca ggcagtgcat ataaagcatg atccagcctg cagtcttcta
      421 gacctcgagc ttcaaactct tcctggtaac gcactggaat ctctatgagg tgcctcaaaa
      481 tgatttttgc catggtgcgg ccgcttttgg atccgagctc ggtaccaagc ttaagtttaa
      541 acgctagagt ccggaggctg gatcggtccc ggtgtcttct atggaggtca aaacagcgtg
      601 gatggcgtct ccaggcgatc tgacggttca ctaaacgagc tcgtcgacga tctctatcac
      661 tgatagggag atctctatca ctgataggga gagctctgct tatatagacc tcccaccgta
      721 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
      781 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
      841 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaa
//
