LOCUS       sequence-107347-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of MBP-PAA-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW4.15
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..49
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gaacaacaac aacaataaca ataacaacaa cctcgggatc gagggaagga tcacaagttt
       61 gtacaaaaaa gcaggcttaa tgtcggttgt cgaagaagcc actgacgacg cgctctaccc
      121 gattgccgtg ctcattgacg agctgcgcaa cgaagatgtc acgttgcgac tgaacagcat
      181 tcgcaaactc tccacaatcg ctttggcgtt gggtgtggag cgcacacgca acgagctcat
      241 ccaatttttg accgacacga tttacgacga agatgaggtt ctgcttgtgc tcgccgagca
      301 actcggaaat ttcactccat tggtcggtgg tccagatcat gttcattgtc ttctccttcc
      361 acttgagaac ttggccacag tcgaggagac cgtggtccgt gacaaagccg ttgagagtct
      421 ccgcaaaatc gccgacaagc acagctcggc ctcattggag gagcatttcg tgccgatgct
      481 cagacgtttg gccactggag actggttcac atcccgtacc tcggcttgtg ggcttttctc
      541 tgttgtttat ccacgtgtca gtccagctat caagtcggaa ttgaagagca tgttccgaac
      601 cctgtgccgt gatgacaccc caatggttcg ccgtgctgct gctgcaaagc tcggagagtt
      661 cgcaaaggtg tttgagaaga ctgccgtcat cgagggtctc cactccagtc tcactgatct
      721 tcatgttgat gagcaagatt ctgttcgttt gctcactgtc gagtcagcca tcgctttcgg
      781 aactctcctc gataaggcta acaagaagaa actcatcgag ccaattctca ttgaattgtt
      841 cgatgacaag tcgtggagag ttcgttatat ggttgccgag aagcttatcg agattcagaa
      901 tgtcctcggt gaggatatgg ataccacaca tctcgttaac atgtacacga accttctcaa
      961 ggatccagag ggtgaggtta gatgtgctgc c
//
